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Title: High sensitivity fluorescent single particle and single molecule detection apparatus and method

Abstract

Apparatus is described for ultrasensitive detection of single fluorescent particles down to the single fluorescent molecule limit in a fluid or on a substrate comprising means for illuminating a predetermined volume of the fluid or area of the substrate whereby to emit light including background light from the fluid and burst of photons from particles residing in the area. The photon burst is detected in real time to generate output representative signal. The signal is received and the burst of energy from the fluorescent particles is distinguished from the background energy to provide an indication of the number, location or concentration of the particles or molecules.

Inventors:
 [1];  [1];  [2]
  1. Contra Costa County, CA
  2. Santa Clara County, CA
Issue Date:
Research Org.:
University of California
OSTI Identifier:
867657
Patent Number(s):
4979824
Assignee:
Board of Trustees of Leland Stanford Junior University (Stanford, CA)
DOE Contract Number:  
FG03-88ER60706
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
sensitivity; fluorescent; single; particle; molecule; detection; apparatus; method; described; ultrasensitive; particles; limit; fluid; substrate; comprising; means; illuminating; predetermined; volume; whereby; emit; light; including; background; burst; photons; residing; photon; detected; time; generate; output; representative; signal; received; energy; distinguished; provide; indication; location; concentration; molecules; substrate comprising; substrate whereby; single particle; sensitive detection; background light; detection apparatus; predetermined volume; single fluorescent; emit light; fluorescent particles; single molecule; generate output; rate output; rate comprising; /356/250/

Citation Formats

Mathies, Richard A, Peck, Konan, and Stryer, Lubert. High sensitivity fluorescent single particle and single molecule detection apparatus and method. United States: N. p., 1990. Web.
Mathies, Richard A, Peck, Konan, & Stryer, Lubert. High sensitivity fluorescent single particle and single molecule detection apparatus and method. United States.
Mathies, Richard A, Peck, Konan, and Stryer, Lubert. Mon . "High sensitivity fluorescent single particle and single molecule detection apparatus and method". United States. https://www.osti.gov/servlets/purl/867657.
@article{osti_867657,
title = {High sensitivity fluorescent single particle and single molecule detection apparatus and method},
author = {Mathies, Richard A and Peck, Konan and Stryer, Lubert},
abstractNote = {Apparatus is described for ultrasensitive detection of single fluorescent particles down to the single fluorescent molecule limit in a fluid or on a substrate comprising means for illuminating a predetermined volume of the fluid or area of the substrate whereby to emit light including background light from the fluid and burst of photons from particles residing in the area. The photon burst is detected in real time to generate output representative signal. The signal is received and the burst of energy from the fluorescent particles is distinguished from the background energy to provide an indication of the number, location or concentration of the particles or molecules.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1990},
month = {1}
}

Patent:

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