High sensitivity fluorescent single particle and single molecule detection apparatus and method
Abstract
Apparatus is described for ultrasensitive detection of single fluorescent particles down to the single fluorescent molecule limit in a fluid or on a substrate comprising means for illuminating a predetermined volume of the fluid or area of the substrate whereby to emit light including background light from the fluid and burst of photons from particles residing in the area. The photon burst is detected in real time to generate output representative signal. The signal is received and the burst of energy from the fluorescent particles is distinguished from the background energy to provide an indication of the number, location or concentration of the particles or molecules.
- Inventors:
-
- Contra Costa County, CA
- Santa Clara County, CA
- Issue Date:
- Research Org.:
- Univ. of California (United States)
- OSTI Identifier:
- 867657
- Patent Number(s):
- 4979824
- Assignee:
- Board of Trustees of Leland Stanford Junior University (Stanford, CA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- FG03-88ER60706
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- sensitivity; fluorescent; single; particle; molecule; detection; apparatus; method; described; ultrasensitive; particles; limit; fluid; substrate; comprising; means; illuminating; predetermined; volume; whereby; emit; light; including; background; burst; photons; residing; photon; detected; time; generate; output; representative; signal; received; energy; distinguished; provide; indication; location; concentration; molecules; substrate comprising; substrate whereby; single particle; sensitive detection; background light; detection apparatus; predetermined volume; single fluorescent; emit light; fluorescent particles; single molecule; generate output; rate output; rate comprising; /356/250/
Citation Formats
Mathies, Richard A, Peck, Konan, and Stryer, Lubert. High sensitivity fluorescent single particle and single molecule detection apparatus and method. United States: N. p., 1990.
Web.
Mathies, Richard A, Peck, Konan, & Stryer, Lubert. High sensitivity fluorescent single particle and single molecule detection apparatus and method. United States.
Mathies, Richard A, Peck, Konan, and Stryer, Lubert. Mon .
"High sensitivity fluorescent single particle and single molecule detection apparatus and method". United States. https://www.osti.gov/servlets/purl/867657.
@article{osti_867657,
title = {High sensitivity fluorescent single particle and single molecule detection apparatus and method},
author = {Mathies, Richard A and Peck, Konan and Stryer, Lubert},
abstractNote = {Apparatus is described for ultrasensitive detection of single fluorescent particles down to the single fluorescent molecule limit in a fluid or on a substrate comprising means for illuminating a predetermined volume of the fluid or area of the substrate whereby to emit light including background light from the fluid and burst of photons from particles residing in the area. The photon burst is detected in real time to generate output representative signal. The signal is received and the burst of energy from the fluorescent particles is distinguished from the background energy to provide an indication of the number, location or concentration of the particles or molecules.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1990},
month = {1}
}
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