skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Methods And Devices For Characterizing Duplex Nucleic Acid Molecules

Abstract

Methods and devices are provided for characterizing a duplex nucleic acid, e.g., a duplex DNA molecule. In the subject methods, a fluid conducting medium that includes a duplex nucleic acid molecule is contacted with a nanopore under the influence of an applied electric field and the resulting changes in current through the nanopore caused by the duplex nucleic acid molecule are monitored. The observed changes in current through the nanopore are then employed as a set of data values to characterize the duplex nucleic acid, where the set of data values may be employed in raw form or manipulated, e.g., into a current blockade profile. Also provided are nanopore devices for practicing the subject methods, where the subject nanopore devices are characterized by the presence of an algorithm which directs a processing means to employ monitored changes in current through a nanopore to characterize a duplex nucleic acid molecule responsible for the current changes. The subject methods and devices find use in a variety of applications, including, among other applications, the identification of an analyte duplex DNA molecule in a sample, the specific base sequence at a single nulceotide polymorphism (SNP), and the sequencing of duplex DNA molecules.

Inventors:
 [1];  [1];  [1];  [1]
  1. Santa Cruz, CA
Issue Date:
Research Org.:
Univ. of California (United States)
OSTI Identifier:
879731
Patent Number(s):
6936433
Application Number:
09/990102
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
FG03-99ER62849
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Akeson, Mark, Vercoutere, Wenonah, Haussler, David, and Winters-Hilt, Stephen. Methods And Devices For Characterizing Duplex Nucleic Acid Molecules. United States: N. p., 2005. Web.
Akeson, Mark, Vercoutere, Wenonah, Haussler, David, & Winters-Hilt, Stephen. Methods And Devices For Characterizing Duplex Nucleic Acid Molecules. United States.
Akeson, Mark, Vercoutere, Wenonah, Haussler, David, and Winters-Hilt, Stephen. Tue . "Methods And Devices For Characterizing Duplex Nucleic Acid Molecules". United States. https://www.osti.gov/servlets/purl/879731.
@article{osti_879731,
title = {Methods And Devices For Characterizing Duplex Nucleic Acid Molecules},
author = {Akeson, Mark and Vercoutere, Wenonah and Haussler, David and Winters-Hilt, Stephen},
abstractNote = {Methods and devices are provided for characterizing a duplex nucleic acid, e.g., a duplex DNA molecule. In the subject methods, a fluid conducting medium that includes a duplex nucleic acid molecule is contacted with a nanopore under the influence of an applied electric field and the resulting changes in current through the nanopore caused by the duplex nucleic acid molecule are monitored. The observed changes in current through the nanopore are then employed as a set of data values to characterize the duplex nucleic acid, where the set of data values may be employed in raw form or manipulated, e.g., into a current blockade profile. Also provided are nanopore devices for practicing the subject methods, where the subject nanopore devices are characterized by the presence of an algorithm which directs a processing means to employ monitored changes in current through a nanopore to characterize a duplex nucleic acid molecule responsible for the current changes. The subject methods and devices find use in a variety of applications, including, among other applications, the identification of an analyte duplex DNA molecule in a sample, the specific base sequence at a single nulceotide polymorphism (SNP), and the sequencing of duplex DNA molecules.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2005},
month = {8}
}

Patent:

Save / Share:

Works referenced in this record:

Characterization of Nucleic Acids by Nanopore Analysis
journal, October 2002


Optimizing planar lipid bilayer single-channel recordings for high resolution with rapid voltage steps
journal, August 1990


Nanopores and nucleic acids: prospects for ultrarapid sequencing
journal, April 2000


Rapid discrimination among individual DNA hairpin molecules at single-nucleotide resolution using an ion channel
journal, March 2001


Characterization of individual polynucleotide molecules using a membrane channel
journal, November 1996