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Title: Using phylogenetic probes for quantification of stable isotope labeling and microbial community analysis

Abstract

Herein is described methods for a high-sensitivity means to measure the incorporation of stable isotope labeled substrates into RNA following stable isotope probing experiments (SIP). RNA is hybridized to a set of probes such as phylogenetic microarrays and isotope incorporation is quantified such as by secondary ion mass spectrometer imaging (NanoSIMS).

Inventors:
; ; ;
Issue Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1165130
Patent Number(s):
8,906,610
Application Number:
13/023,538
Assignee:
The Regents of the University of California (Oakland, CA)
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2011 Feb 08
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Brodie, Eoin L, DeSantis, Todd Z, Karaoz, Ulas, and Andersen, Gary L. Using phylogenetic probes for quantification of stable isotope labeling and microbial community analysis. United States: N. p., 2014. Web.
Brodie, Eoin L, DeSantis, Todd Z, Karaoz, Ulas, & Andersen, Gary L. Using phylogenetic probes for quantification of stable isotope labeling and microbial community analysis. United States.
Brodie, Eoin L, DeSantis, Todd Z, Karaoz, Ulas, and Andersen, Gary L. Tue . "Using phylogenetic probes for quantification of stable isotope labeling and microbial community analysis". United States. https://www.osti.gov/servlets/purl/1165130.
@article{osti_1165130,
title = {Using phylogenetic probes for quantification of stable isotope labeling and microbial community analysis},
author = {Brodie, Eoin L and DeSantis, Todd Z and Karaoz, Ulas and Andersen, Gary L},
abstractNote = {Herein is described methods for a high-sensitivity means to measure the incorporation of stable isotope labeled substrates into RNA following stable isotope probing experiments (SIP). RNA is hybridized to a set of probes such as phylogenetic microarrays and isotope incorporation is quantified such as by secondary ion mass spectrometer imaging (NanoSIMS).},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {12}
}

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