Using phylogenetic probes for quantification of stable isotope labeling and microbial community analysis
Abstract
Herein is described methods for a high-sensitivity means to measure the incorporation of stable isotope labeled substrates into RNA following stable isotope probing experiments (SIP). RNA is hybridized to a set of probes such as phylogenetic microarrays and isotope incorporation is quantified such as by secondary ion mass spectrometer imaging (NanoSIMS).
- Inventors:
- Issue Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1165130
- Patent Number(s):
- 8906610
- Application Number:
- 13/023,538
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- AC02-05CH11231
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2011 Feb 08
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Brodie, Eoin L, DeSantis, Todd Z, Karaoz, Ulas, and Andersen, Gary L. Using phylogenetic probes for quantification of stable isotope labeling and microbial community analysis. United States: N. p., 2014.
Web.
Brodie, Eoin L, DeSantis, Todd Z, Karaoz, Ulas, & Andersen, Gary L. Using phylogenetic probes for quantification of stable isotope labeling and microbial community analysis. United States.
Brodie, Eoin L, DeSantis, Todd Z, Karaoz, Ulas, and Andersen, Gary L. Tue .
"Using phylogenetic probes for quantification of stable isotope labeling and microbial community analysis". United States. https://www.osti.gov/servlets/purl/1165130.
@article{osti_1165130,
title = {Using phylogenetic probes for quantification of stable isotope labeling and microbial community analysis},
author = {Brodie, Eoin L and DeSantis, Todd Z and Karaoz, Ulas and Andersen, Gary L},
abstractNote = {Herein is described methods for a high-sensitivity means to measure the incorporation of stable isotope labeled substrates into RNA following stable isotope probing experiments (SIP). RNA is hybridized to a set of probes such as phylogenetic microarrays and isotope incorporation is quantified such as by secondary ion mass spectrometer imaging (NanoSIMS).},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {12}
}
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