Method to detect the end-point for PCR DNA amplification using an ionically labeled probe and measuring impedance change
Abstract
Impedance measurements are used to detect the end-point for PCR DNA amplification. A pair of spaced electrodes are located on a surface of a microfluidic channel and an AC or DC voltage is applied across the electrodes to produce an electric field. An ionically labeled probe will attach to a complementary DNA segment, and a polymerase enzyme will release the ionic label. This causes the conductivity of the solution in the area of the electrode to change. This change in conductivity is measured as a change in the impedance been the two electrodes.
- Inventors:
-
- Danville, CA
- Severna Park, MD
- Oakland, CA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 903351
- Patent Number(s):
- 7157232
- Application Number:
- 11/020,731
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Miles, Robin R, Belgrader, Phillip, and Fuller, Christopher D. Method to detect the end-point for PCR DNA amplification using an ionically labeled probe and measuring impedance change. United States: N. p., 2007.
Web.
Miles, Robin R, Belgrader, Phillip, & Fuller, Christopher D. Method to detect the end-point for PCR DNA amplification using an ionically labeled probe and measuring impedance change. United States.
Miles, Robin R, Belgrader, Phillip, and Fuller, Christopher D. Tue .
"Method to detect the end-point for PCR DNA amplification using an ionically labeled probe and measuring impedance change". United States. https://www.osti.gov/servlets/purl/903351.
@article{osti_903351,
title = {Method to detect the end-point for PCR DNA amplification using an ionically labeled probe and measuring impedance change},
author = {Miles, Robin R and Belgrader, Phillip and Fuller, Christopher D},
abstractNote = {Impedance measurements are used to detect the end-point for PCR DNA amplification. A pair of spaced electrodes are located on a surface of a microfluidic channel and an AC or DC voltage is applied across the electrodes to produce an electric field. An ionically labeled probe will attach to a complementary DNA segment, and a polymerase enzyme will release the ionic label. This causes the conductivity of the solution in the area of the electrode to change. This change in conductivity is measured as a change in the impedance been the two electrodes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 02 00:00:00 EST 2007},
month = {Tue Jan 02 00:00:00 EST 2007}
}
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Works referenced in this record:
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