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Title: Quantitative substrate utilization in microbial ecology using stable isotope probing

Abstract

Methods modifying conventional SIP so that isotopic incorporation into the genomes of individual microbial taxa can be quantified are described. Further, methods to quantify the baseline densities of the DNA of individual microbial taxa without exposure to isotope tracers and then to quantify the change in DNA density of each taxon caused by isotope incorporation are described. The distribution of DNA of each taxon along a density gradient reflects the influence of isotope incorporation only, without reflecting the guanine-plus-cytosine content.

Inventors:
; ; ;
Issue Date:
Research Org.:
Northern Arizona Univ., Flagstaff, AZ (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1600267
Patent Number(s):
10472670
Application Number:
15/640,028
Assignee:
Arizona Board of Regents on Behalf of Northern Arizona University (Flagstaff, AZ)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
SC0010579
Resource Type:
Patent
Resource Relation:
Patent File Date: 06/30/2017
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY

Citation Formats

Hungate, Bruce, Schwartz, Egbert, Mau, Rebecca, and Marks, Jane. Quantitative substrate utilization in microbial ecology using stable isotope probing. United States: N. p., 2019. Web.
Hungate, Bruce, Schwartz, Egbert, Mau, Rebecca, & Marks, Jane. Quantitative substrate utilization in microbial ecology using stable isotope probing. United States.
Hungate, Bruce, Schwartz, Egbert, Mau, Rebecca, and Marks, Jane. Tue . "Quantitative substrate utilization in microbial ecology using stable isotope probing". United States. https://www.osti.gov/servlets/purl/1600267.
@article{osti_1600267,
title = {Quantitative substrate utilization in microbial ecology using stable isotope probing},
author = {Hungate, Bruce and Schwartz, Egbert and Mau, Rebecca and Marks, Jane},
abstractNote = {Methods modifying conventional SIP so that isotopic incorporation into the genomes of individual microbial taxa can be quantified are described. Further, methods to quantify the baseline densities of the DNA of individual microbial taxa without exposure to isotope tracers and then to quantify the change in DNA density of each taxon caused by isotope incorporation are described. The distribution of DNA of each taxon along a density gradient reflects the influence of isotope incorporation only, without reflecting the guanine-plus-cytosine content.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {11}
}

Works referenced in this record:

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journal, October 2016


Stable-isotope probing of nucleic acids: a window to the function of uncultured microorganisms
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Methodological Considerations for the Use of Stable Isotope Probing in Microbial Ecology
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Quantitative Microbial Ecology through Stable Isotope Probing
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Characterization of Growing Microorganisms in Soil by Stable Isotope Probing with H218O
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Identification of Nitrogen-Incorporating Bacteria in Petroleum-Contaminated Arctic Soils by Using [ 15 N]DNA-Based Stable Isotope Probing and Pyrosequencing
journal, April 2011


Cellulose utilization in forest litter and soil: identification of bacterial and fungal decomposers
journal, March 2012


Stable Isotope Probing with 15N Achieved by Disentangling the Effects of Genome G+C Content and Isotope Enrichment on DNA Density
journal, March 2007