Quantitative substrate utilization in microbial ecology using stable isotope probing
Abstract
Methods modifying conventional SIP so that isotopic incorporation into the genomes of individual microbial taxa can be quantified are described. Further, methods to quantify the baseline densities of the DNA of individual microbial taxa without exposure to isotope tracers and then to quantify the change in DNA density of each taxon caused by isotope incorporation are described. The distribution of DNA of each taxon along a density gradient reflects the influence of isotope incorporation only, without reflecting the guanine-plus-cytosine content.
- Inventors:
- Issue Date:
- Research Org.:
- Northern Arizona Univ., Flagstaff, AZ (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1600267
- Patent Number(s):
- 10472670
- Application Number:
- 15/640,028
- Assignee:
- Arizona Board of Regents on Behalf of Northern Arizona University (Flagstaff, AZ)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- SC0010579
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 06/30/2017
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
Citation Formats
Hungate, Bruce, Schwartz, Egbert, Mau, Rebecca, and Marks, Jane. Quantitative substrate utilization in microbial ecology using stable isotope probing. United States: N. p., 2019.
Web.
Hungate, Bruce, Schwartz, Egbert, Mau, Rebecca, & Marks, Jane. Quantitative substrate utilization in microbial ecology using stable isotope probing. United States.
Hungate, Bruce, Schwartz, Egbert, Mau, Rebecca, and Marks, Jane. Tue .
"Quantitative substrate utilization in microbial ecology using stable isotope probing". United States. https://www.osti.gov/servlets/purl/1600267.
@article{osti_1600267,
title = {Quantitative substrate utilization in microbial ecology using stable isotope probing},
author = {Hungate, Bruce and Schwartz, Egbert and Mau, Rebecca and Marks, Jane},
abstractNote = {Methods modifying conventional SIP so that isotopic incorporation into the genomes of individual microbial taxa can be quantified are described. Further, methods to quantify the baseline densities of the DNA of individual microbial taxa without exposure to isotope tracers and then to quantify the change in DNA density of each taxon caused by isotope incorporation are described. The distribution of DNA of each taxon along a density gradient reflects the influence of isotope incorporation only, without reflecting the guanine-plus-cytosine content.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {11}
}
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