Title: Enhancing the sensitivity of immunoassay procedures by use of antibodies directed to the product of a reaction between probe labels and assay substrates

Abstract

The subject invention provides an antibody which specifically binds to the product of a reaction between a labeling substance and a substrate. The subject invention also provides a method of making an immunogen used to produce the antibody of the subject invention. The invention further provides methods of using the subject antibody for detecting an antigen of interest in a sample, for example detecting a protein comprising an amino acid sequence of interest and detecting a nucleic acid molecule comprising a nucleic acid sequence of interest.

Inventors:

Erlanger, Bernard F ^[1]; Chen, Bi-Xing ^[2]

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1. Whitestone, NY
2. New York, NY

Issue Date:

Wed Jan 01 00:00:00 EST 1997

Research Org.:

University of Vermont

OSTI Identifier:

871059

Patent Number(s):

5650284

Assignee:

Trustees of Columbia University in City of New York (New York, NY)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K16/40 - against enzymes
C07K16/44 - against material not provided for elsewhere {, e.g. haptens, metals, DNA, RNA, amino acids}

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6804 - Nucleic acid analysis using immunogens

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/581 - {with enzyme label

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

Y10S435/966 - involving an enzyme system with high turnover rate or complement magnified assay, e.g. multi-enzyme systems

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DOE Contract Number:  

FG02-88ER60742

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

enhancing; sensitivity; immunoassay; procedures; antibodies; directed; product; reaction; probe; labels; assay; substrates; subject; provides; antibody; specifically; binds; labeling; substance; substrate; method; immunogen; produce; methods; detecting; antigen; sample; example; protein; comprising; amino; acid; sequence; nucleic; molecule; acid molecule; provides methods; acid sequence; amino acid; nucleic acid; specifically bind; molecule comprising; specifically binds; immunoassay procedures; probe labels; antibodies directed; assay substrates; /435/530/