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Title: Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation

Abstract

Embodiments of the present invention are directed toward devices, systems, and method for conducting assays using sedimentation. In one example, a method includes layering a mixture on a density medium, subjecting sedimentation particles in the mixture to sedimentation forces to cause the sedimentation particles to move to a detection area through a density medium, and detecting a target analyte in a detection region of the sedimentation channel. In some examples, the sedimentation particles and labeling agent may have like charges to reduce non-specific binding of labeling agent and sedimentation particles. In some examples, the density medium is provided with a separation layer for stabilizing the assay during storage and operation. In some examples, the sedimentation channel may be provided with a generally flat sedimentation chamber for dispersing the particle pellet over a larger surface area.

Inventors:
; ;
Issue Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1171088
Patent Number(s):
8962346
Application Number:
13/423,050
Assignee:
Sandia Corporation (Albuquerque, NM)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Mar 16
Country of Publication:
United States
Language:
English
Subject:
42 ENGINEERING

Citation Formats

Schaff, Ulrich Y, Koh, Chung-Yan, and Sommer, Gregory J. Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation. United States: N. p., 2015. Web.
Schaff, Ulrich Y, Koh, Chung-Yan, & Sommer, Gregory J. Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation. United States.
Schaff, Ulrich Y, Koh, Chung-Yan, and Sommer, Gregory J. Tue . "Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation". United States. https://www.osti.gov/servlets/purl/1171088.
@article{osti_1171088,
title = {Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation},
author = {Schaff, Ulrich Y and Koh, Chung-Yan and Sommer, Gregory J},
abstractNote = {Embodiments of the present invention are directed toward devices, systems, and method for conducting assays using sedimentation. In one example, a method includes layering a mixture on a density medium, subjecting sedimentation particles in the mixture to sedimentation forces to cause the sedimentation particles to move to a detection area through a density medium, and detecting a target analyte in a detection region of the sedimentation channel. In some examples, the sedimentation particles and labeling agent may have like charges to reduce non-specific binding of labeling agent and sedimentation particles. In some examples, the density medium is provided with a separation layer for stabilizing the assay during storage and operation. In some examples, the sedimentation channel may be provided with a generally flat sedimentation chamber for dispersing the particle pellet over a larger surface area.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {2}
}

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Works referenced in this record:

A molecular approach to bioseparations: Protein–protein and protein–salt interactions
journal, February 2006


Infrared controlled waxes for liquid handling and storage on a CD-microfluidic platform
journal, January 2011


How Hofmeister ion interactions affect protein stability
journal, October 1996


Cell-Free DNA—A Marker to Predict Ischemic Brain Damage in a Rat Stroke Experimental Model
journal, January 2011


Measurement of Circulating Cell-Free DNA Levels by a New Simple Fluorescent Test in Patients With Primary Colorectal Cancer
journal, February 2011


A rapid direct fluorescent assay for cell-free DNA quantification in biological fluids
journal, September 2009


Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry
journal, January 2009


A fully automated immunoassay from whole blood on a disc
journal, January 2009


Bead-based microfluidic immunoassays: The next generation
journal, February 2007


Lab On A CD
journal, August 2006


Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry
journal, January 2007


Functional integration of DNA purification and concentration into a real time micro-PCR chip
journal, January 2011


Plasma DNA concentration as a predictor of mortality and sepsis in critically ill patients
journal, April 2006


A B Cell-Based Sensor for Rapid Identification of Pathogens
journal, July 2003


Read-out concepts for multiplexed bead-based fluorescence immunoassays on centrifugal microfluidic platforms
journal, February 2006


Whole Blood Immunoassay Based on Centrifugal Bead Sedimentation
journal, March 2011


A new biodosimetric method: branched DNA-based quantitative detection of B1 DNA in mouse plasma
journal, August 2010


Circulating DNA: a new diagnostic gold mine?
journal, October 2002


Staphylococcal Enterotoxin B as a Biological Weapon: Recognition, Management, and Surveillance of Staphylococcal Enterotoxin
journal, September 2006


Ricin A-Chain Activity on Stem−Loop and Unstructured DNA Substrates
journal, March 2005


Parallel Nanoliter Microfluidic Analysis System
journal, June 2007


One-step purification of nucleic acid for gene expression analysis via Immiscible Filtration Assisted by Surface Tension (IFAST)
journal, January 2011


Shiga toxin 1 acting on DNA in vitro is a heat-stable enzyme not requiring proteolytic activation
journal, April 2004


Fully integrated lab-on-a-disc for simultaneous analysis of biochemistry and immunoassay from whole blood
journal, January 2011


Cell-Free Plasma DNA as a Predictor of Outcome in Severe Sepsis and Septic Shock
journal, April 2008


Bioinformatic processing to identify single nucleotide polymorphism that potentially affect Ape1 function
journal, June 2011


Micro freef-low IEF enhanced by active cooling and functionalized gels
journal, December 2006


Formation of Natural pH Gradients in a Microfluidic Device under Flow Conditions:  Model and Experimental Validation
journal, February 2001


Multistage Isoelectric Focusing in a Polymeric Microfluidic Chip
journal, December 2005


Effects of separation length and voltage on isoelectric focusing in a plastic microfluidic device
journal, September 2006


Detecting Single Stranded DNA with a Solid State Nanopore
journal, October 2005


The current state of two-dimensional electrophoresis with immobilized pH gradients
journal, April 2000


Integrated Preconcentration SDS−PAGE of Proteins in Microchips Using Photopatterned Cross-Linked Polyacrylamide Gels
journal, July 2006


Microfluidic immunoassays as rapid saliva-based clinical diagnostics
journal, March 2007


On-Chip Coupling of Isoelectric Focusing and Free Solution Electrophoresis for Multidimensional Separations
journal, March 2003


Rapid isoelectric trapping in a micropreparative-scale multicompartment electrolyzer
journal, June 2007


Photopolymerized diffusion-defined polyacrylamide gradient gels for on-chip protein sizing
journal, January 2008


Integration of nanoporous membranes for sample filtration/preconcentration in microchip electrophoresis
journal, December 2006


Preparative-scale isoelectric trapping separations using a modified Gradiflow unit
journal, December 2002


Design Optimization of Spirally Grooved Thrust Air Bearings for Polygon Mirror Laser Scanners
journal, January 1993


On-Chip Isoelectric Focusing Using Photopolymerized Immobilized pH Gradients
journal, May 2008


Miniaturized capillary isoelectric focusing in plastic microfluidic devices
journal, October 2002


Parallel isoelectric focusing chip
journal, September 2004


Parallel isoelectric focusing II
journal, November 2004


Parallel processing in the isoelectric focusing chip
journal, November 2003