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Title: Microelectroporation device for genomic screening

Abstract

We have developed an microelectroporation device that combines microarrays of oligonucleotides, microfluidic channels, and electroporation for cell transfection and high-throughput screening applications (e.g. RNA interference screens). Microarrays allow the deposition of thousands of different oligonucleotides in microscopic spots. Microfluidic channels and microwells enable efficient loading of cells into the device and prevent cross-contamination between different oligonucleotides spots. Electroporation allows optimal transfection of nucleic acids into cells (especially hard-to-transfect cells such as primary cells) by minimizing cell death while maximizing transfection efficiency. This invention has the advantage of a higher throughput and lower cost, while preventing cross-contamination compared to conventional screening technologies. Moreover, this device does not require bulky robotic liquid handling equipment and is inherently safer given that it is a closed system.

Inventors:
; ; ;
Issue Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1157509
Patent Number(s):
8,828,736
Application Number:
13/173,180
Assignee:
Sandia Corporation (Albuquerque, NM)
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Perroud, Thomas D., Renzi, Ronald F., Negrete, Oscar, and Claudnic, Mark R. Microelectroporation device for genomic screening. United States: N. p., 2014. Web.
Perroud, Thomas D., Renzi, Ronald F., Negrete, Oscar, & Claudnic, Mark R. Microelectroporation device for genomic screening. United States.
Perroud, Thomas D., Renzi, Ronald F., Negrete, Oscar, and Claudnic, Mark R. Tue . "Microelectroporation device for genomic screening". United States. https://www.osti.gov/servlets/purl/1157509.
@article{osti_1157509,
title = {Microelectroporation device for genomic screening},
author = {Perroud, Thomas D. and Renzi, Ronald F. and Negrete, Oscar and Claudnic, Mark R.},
abstractNote = {We have developed an microelectroporation device that combines microarrays of oligonucleotides, microfluidic channels, and electroporation for cell transfection and high-throughput screening applications (e.g. RNA interference screens). Microarrays allow the deposition of thousands of different oligonucleotides in microscopic spots. Microfluidic channels and microwells enable efficient loading of cells into the device and prevent cross-contamination between different oligonucleotides spots. Electroporation allows optimal transfection of nucleic acids into cells (especially hard-to-transfect cells such as primary cells) by minimizing cell death while maximizing transfection efficiency. This invention has the advantage of a higher throughput and lower cost, while preventing cross-contamination compared to conventional screening technologies. Moreover, this device does not require bulky robotic liquid handling equipment and is inherently safer given that it is a closed system.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {9}
}

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