Microelectroporation device for genomic screening
Abstract
We have developed an microelectroporation device that combines microarrays of oligonucleotides, microfluidic channels, and electroporation for cell transfection and high-throughput screening applications (e.g. RNA interference screens). Microarrays allow the deposition of thousands of different oligonucleotides in microscopic spots. Microfluidic channels and microwells enable efficient loading of cells into the device and prevent cross-contamination between different oligonucleotides spots. Electroporation allows optimal transfection of nucleic acids into cells (especially hard-to-transfect cells such as primary cells) by minimizing cell death while maximizing transfection efficiency. This invention has the advantage of a higher throughput and lower cost, while preventing cross-contamination compared to conventional screening technologies. Moreover, this device does not require bulky robotic liquid handling equipment and is inherently safer given that it is a closed system.
- Inventors:
- Issue Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1157509
- Patent Number(s):
- 8828736
- Application Number:
- 13/173,180
- Assignee:
- Sandia Corporation (Albuquerque, NM)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
C - CHEMISTRY C12 - BIOCHEMISTRY C12M - APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
- DOE Contract Number:
- AC04-94AL85000
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Perroud, Thomas D., Renzi, Ronald F., Negrete, Oscar, and Claudnic, Mark R. Microelectroporation device for genomic screening. United States: N. p., 2014.
Web.
Perroud, Thomas D., Renzi, Ronald F., Negrete, Oscar, & Claudnic, Mark R. Microelectroporation device for genomic screening. United States.
Perroud, Thomas D., Renzi, Ronald F., Negrete, Oscar, and Claudnic, Mark R. Tue .
"Microelectroporation device for genomic screening". United States. https://www.osti.gov/servlets/purl/1157509.
@article{osti_1157509,
title = {Microelectroporation device for genomic screening},
author = {Perroud, Thomas D. and Renzi, Ronald F. and Negrete, Oscar and Claudnic, Mark R.},
abstractNote = {We have developed an microelectroporation device that combines microarrays of oligonucleotides, microfluidic channels, and electroporation for cell transfection and high-throughput screening applications (e.g. RNA interference screens). Microarrays allow the deposition of thousands of different oligonucleotides in microscopic spots. Microfluidic channels and microwells enable efficient loading of cells into the device and prevent cross-contamination between different oligonucleotides spots. Electroporation allows optimal transfection of nucleic acids into cells (especially hard-to-transfect cells such as primary cells) by minimizing cell death while maximizing transfection efficiency. This invention has the advantage of a higher throughput and lower cost, while preventing cross-contamination compared to conventional screening technologies. Moreover, this device does not require bulky robotic liquid handling equipment and is inherently safer given that it is a closed system.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Sep 09 00:00:00 EDT 2014},
month = {Tue Sep 09 00:00:00 EDT 2014}
}
Works referenced in this record:
Protein Crystallization Droplet Actuator, System and Method
patent-application, February 2008
- Pamula, Vamsee K.; Srinivasan, Vijay; Pollack, Michael G.
- US Patent Application 11/839762; 20080050834