Title: Engineered microorganisms capable of producing target compounds under anaerobic conditions

Abstract

The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.

Inventors:

Buelter, Thomas ^[1]; Meinhold, Peter ^[1]; Feldman, Reid M. Renny ^[2]; Hawkins, Andrew C ^[3]; Urano, Jun ^[4]; Bastian, Sabine ^[5]; Arnold, Frances ^[6]

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+ Show Inventor Affiliations

1. Denver, CO
2. San Francisco, CA
3. Parker, CO
4. Irvine, CA
5. Pasadena, CA
6. La Canada, CA

Issue Date:

Tue Jan 17 00:00:00 EST 2012

Research Org.:

Gevo, Inc. (Englewood, CO)

Sponsoring Org.:

USDOE

OSTI Identifier:

1034914

Patent Number(s):

8097440

Application Number:

13/269,937

Assignee:

Gevo, Inc. (Englewood, CO); California Institute of Technology (Pasadena, CA)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/0006 - {acting on CH-OH groups as donors
C12N9/0008 - {acting on the aldehyde or oxo group of donors
C12N9/0036 - {acting on NADH or NADPH

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P7/16 - Butanols

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

Y02E50/10 - Biofuels

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DOE Contract Number:  

FG02-07ER84893

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

60 APPLIED LIFE SCIENCES