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Title: Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702

Abstract

Bacteria which produce large amounts of cellulose-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

Inventors:
 [1]
  1. (Lenoir City, TN)
Issue Date:
Research Org.:
LOCKHEED MARTIN ENRGY SYST INC
OSTI Identifier:
871571
Patent Number(s):
5756337
Assignee:
Lockheed Martin Energy Systems, Inc. (Oak Ridge, TN) ORNL
DOE Contract Number:  
AC05-84OR21400
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
method; producing; cellulase-containing; cell-free; fermentate; produced; microorganism; atcc; 55702; bacteria; produce; amounts; cellulose-containing; identified; original; bacterium; 55703; genetically; altered; nitrosoguanidine; mnng; treatment; enhanced; cellulase; replicate; plating; improved; characteristics; qualities; degradation; cellulosic; waste; materials; fuel; production; food; processing; textile; industrial; applications; bacterial; host; genetic; manipulations; recombinant; dna; techniques; destroy; standard; mutagenesis; cellulase producing; cellulase-containing cell-free; industrial applications; improved characteristics; original bacterium; bacterial host; enhanced cellulase; replicate plating; waste material; waste materials; recombinant dna; genetically altered; cell-free fermentate; genetic manipulations; standard mutagenesis; microorganism atcc; mutagenesis techniques; fuel production; textile processing; fermentate produced; dna techniques; destroy genetic; improved bacterial; improved bacteria; food processing; cellulosic waste; producing bacterium; fuel product; /435/

Citation Formats

Dees, H. Craig. Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702. United States: N. p., 1998. Web.
Dees, H. Craig. Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702. United States.
Dees, H. Craig. Thu . "Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702". United States. https://www.osti.gov/servlets/purl/871571.
@article{osti_871571,
title = {Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702},
author = {Dees, H. Craig},
abstractNote = {Bacteria which produce large amounts of cellulose-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}

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