Global to push GA events into
skip to main content

Title: Microorganisms and methods for producing pyruvate, ethanol, and other compounds

Microorganisms comprising modifications for producing pyruvate, ethanol, and other compounds. The microorganisms comprise modifications that reduce or ablate activity of one or more of pyruvate dehydrogenase, 2-oxoglutarate dehydrogenase, phosphate acetyltransferase, acetate kinase, pyruvate oxidase, lactate dehydrogenase, cytochrome terminal oxidase, succinate dehydrogenase, 6-phosphogluconate dehydrogenase, glutamate dehydrogenase, pyruvate formate lyase, pyruvate formate lyase activating enzyme, and isocitrate lyase. The microorganisms optionally comprise modifications that enhance expression or activity of pyruvate decarboxylase and alcohol dehydrogenase. The microorganisms are optionally evolved in defined media to enhance specific production of one or more compounds. Methods of producing compounds with the microorganisms are provided.
Issue Date:
OSTI Identifier:
Patent Number(s):
Application Number:
Contract Number:
FC02-07ER64494; SC0008103
Resource Relation:
Patent File Date: 2015 Sep 09
Research Org:
Wisconsin Alumni Research Foundation, Madison, WI (United States)
Sponsoring Org:
Country of Publication:
United States

Works referenced in this record:

Basic local alignment search tool
journal, October 1990
  • Altschul, Stephen F.; Gish, Warren; Miller, Webb
  • Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
  • DOI: 10.1016/S0022-2836(05)80360-2

Enhancing sesquiterpene production in Saccharomyces cerevisiae through in silico driven metabolic engineering
journal, November 2009
  • Asadollahi, Mohammad A.; Maury, Jérôme; Patil, Kiran Raosaheb
  • Metabolic Engineering, Vol. 11, Issue 6, p. 328-334
  • DOI: 10.1016/j.ymben.2009.07.001

Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
journal, January 2008
  • Atsumi, Shota; Hanai, Taizo; Liao, James C.
  • Nature, Vol. 451, Issue 7174, p. 86-89
  • DOI: 10.1038/nature06450

Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants the Keio collection
journal, February 2006
  • Baba, Tomoya; Ara, Takeshi; Hasegawa, Miki
  • Molecular Systems Biology, Vol. 2, Article No. 2006.0008
  • DOI: 10.1038/msb4100050

The evolution of metabolic networks of E. coli
journal, January 2011
  • Baumler, David J.; Peplinski, Roman G.; Reed, Jennifer L.
  • BMC Systems Biology, Vol. 5, Issue 1, Article No. 182
  • DOI: 10.1186/1752-0509-5-182

Genes Involved in Long-Chain Alkene Biosynthesis in Micrococcus luteus
journal, December 2009
  • Beller, H. R.; Goh, E.-B.; Keasling, J. D.
  • Applied and Environmental Microbiology, Vol. 76, Issue 4, p. 1212-1223
  • DOI: 10.1128/AEM.02312-09

Characterization of Escherichia coli EutD: a phosphotransacetylase of the ethanolamine operon
journal, October 2010
  • Bologna, Federico P.; Campos-Bermudez, Valeria A.; Saavedra, Damián D.
  • The Journal of Microbiology, Vol. 48, Issue 5, p. 629-636
  • DOI: 10.1007/s12275-010-0091-0

Engineering Escherichia coli for efficient conversion of glucose to pyruvate
journal, February 2004
  • Causey, T. B.; Shanmugam, K. T.; Yomano, L. P.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 8, p. 2235-2240
  • DOI: 10.1073/pnas.0308171100

Engineering the metabolism of Escherichia coli W3110 for the conversion of sugar to redox-neutral and oxidized products: Homoacetate production
journal, January 2003
  • Causey, T. B.; Zhou, S.; Shanmugam, K. T.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 3, p. 825-832
  • DOI: 10.1073/pnas.0337684100

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
journal, May 2000
  • Datsenko, K. A.; Wanner, B. L.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 12, p. 6640-6645
  • DOI: 10.1073/pnas.120163297

Model-driven evaluation of the production potential for growth-coupled products of Escherichia coli
journal, May 2010
  • Feist, Adam M.; Zielinski, Daniel C.; Orth, Jeffrey D.
  • Metabolic Engineering, Vol. 12, Issue 3, p. 173-186
  • DOI: 10.1016/j.ymben.2009.10.003

In silico design and adaptive evolution of Escherichia coli for production of lactic acid
journal, September 2005
  • Fong, Stephen S.; Burgard, Anthony P.; Herring, Christopher D.
  • Biotechnology and Bioengineering, Vol. 91, Issue 5, p. 643-648
  • DOI: 10.1002/bit.20542

Production of benzylisoquinoline alkaloids in Saccharomyces cerevisiae
journal, August 2008
  • Hawkins, Kristy M; Smolke, Christina D
  • Nature Chemical Biology, Vol. 4, Issue 9, p. 564-573
  • DOI: 10.1038/nchembio.105

OptORF: Optimal metabolic and regulatory perturbations for metabolic engineering of microbial strains
journal, January 2010
  • Kim, Joonhoon; Reed, Jennifer L.
  • BMC Systems Biology, Vol. 4, Issue 1, Article No. 53
  • DOI: 10.1186/1752-0509-4-53

Initiation of translation in prokaryotes and eukaryotes
journal, July 1999

Combining metabolic and protein engineering of a terpenoid biosynthetic pathway for overproduction and selectivity control
journal, July 2010
  • Leonard, E.; Ajikumar, P. K.; Thayer, K.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 31, p. 13654-13659
  • DOI: 10.1073/pnas.1006138107

Cellulosic hydrolysate toxicity and tolerance mechanisms in Escherichia coli
journal, January 2009
  • Mills, Tirzah Y.; Sandoval, Nicholas R.; Gill, Ryan T.
  • Biotechnology for Biofuels, Vol. 2, Issue 1, Article No. 26
  • DOI: 10.1186/1754-6834-2-26

Metabolic engineering for the microbial production of 1,3-propanediol
journal, October 2003

Metabolic engineering of Escherichia coli for the production of L-valine based on transcriptome analysis and in silico gene knockout simulation
journal, April 2007
  • Park, J. H.; Lee, K. H.; Kim, T. Y.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 19, p. 7797-7802
  • DOI: 10.1073/pnas.0702609104

Global metabolic regulation analysis for Escherichia coli K12 based on protein expression by 2-dimensional electrophoresis and enzyme activity measurement
journal, January 2003

Biosynthesis of Complex Polyketides in a Metabolically Engineered Strain of E. coli
journal, March 2001
  • Pfeifer, Blaine A.; Admiraal, Suzanne J.; Gramajo, Hugo
  • Science, Vol. 291, Issue 5509, p. 1790-1792
  • DOI: 10.1126/science.1058092

Production of the antimalarial drug precursor artemisinic acid in engineered yeast
journal, April 2006
  • Ro, Dae-Kyun; Paradise, Eric M.; Ouellet, Mario
  • Nature, Vol. 440, Issue 7086, p. 940-943
  • DOI: 10.1038/nature04640

A glycyl radical solution: oxygen-dependent interconversion of pyruvate formate-lyase
journal, August 1998

Microbial Biosynthesis of Alkanes
journal, July 2010

Complex Physiology and Compound Stress Responses during Fermentation of Alkali-Pretreated Corn Stover Hydrolysate by an Escherichia coli Ethanologen
journal, March 2012
  • Schwalbach, Michael S.; Keating, David H.; Tremaine, Mary
  • Applied and Environmental Microbiology, Vol. 78, Issue 9, p. 3442-3457
  • DOI: 10.1128/AEM.07329-11

Implementation of communication-mediating domains for non-ribosomal peptide production in Saccharomyces cerevisiae
journal, March 2010
  • Siewers, Verena; San-Bento, Rita; Nielsen, Jens
  • Biotechnology and Bioengineering, Vol. 106, Issue 5, p. 841-844
  • DOI: 10.1002/bit.22739

Microbial production of fatty-acid-derived fuels and chemicals from plant biomass
journal, January 2010
  • Steen, Eric J.; Kang, Yisheng; Bokinsky, Gregory
  • Nature, Vol. 463, Issue 7280, p. 559-562
  • DOI: 10.1038/nature08721

The effect of acetate pathway mutations on the production of pyruvate in Escherichia coli
journal, July 2003
  • Tomar, A.; Eiteman, M. A.; Altman, E.
  • Applied Microbiology and Biotechnology, Vol. 62, Issue 1, p. 76-82
  • DOI: 10.1007/s00253-003-1234-6

Metabolic regulation analysis of wild-type and arcA mutant Escherichia coli under nitrate conditions using different levels of omics data
journal, January 2012
  • Toya, Yoshihiro; Nakahigashi, Kenji; Tomita, Masaru
  • Molecular BioSystems, Vol. 8, Issue 10, p. 2593-2604
  • DOI: 10.1039/c2mb25069a

Production of pyruvate in Saccharomyces cerevisiae through adaptive evolution and rational cofactor metabolic engineering
journal, August 2012

Engineering of Solvent-Tolerant Pseudomonas putida S12 for Bioproduction of Phenol from Glucose
journal, December 2005
  • Wierckx, N. J. P.; Ballerstedt, H.; de Bont, J. A. M.
  • Applied and Environmental Microbiology, Vol. 71, Issue 12, p. 8221-8227
  • DOI: 10.1128/AEM.71.12.8221-8227.2005

Engineering Corynebacterium glutamicum for the production of pyruvate
journal, January 2012
  • Wieschalka, Stefan; Blombach, Bastian; Eikmanns, Bernhard J.
  • Applied Microbiology and Biotechnology, Vol. 94, Issue 2, p. 449-459
  • DOI: 10.1007/s00253-011-3843-9

Regulation of thiamine synthesis in Saccharomyces cerevisiae for improved pyruvate production
journal, June 2012
  • Xu, Guoqiang; Hua, Qiang; Duan, Ningjun
  • Yeast, Vol. 29, Issue 6, p. 209-217
  • DOI: 10.1002/yea.2902

Improving cellular malonyl-CoA level in Escherichia coli via metabolic engineering
journal, May 2009
  • Zha, Wenjuan; Rubin-Pitel, Sheryl B.; Shao, Zengyi
  • Metabolic Engineering, Vol. 11, Issue 3, p. 192-198
  • DOI: 10.1016/j.ymben.2009.01.005

Metabolic evolution of energy-conserving pathways for succinate production in Escherichia coli
journal, November 2009
  • Zhang, X.; Jantama, K.; Moore, J. C.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 48, p. 20180-20185
  • DOI: 10.1073/pnas.0905396106

Global analysis of gene transcription regulation in prokaryotes
journal, August 2006

Evaluation of Genetic Manipulation Strategies on d-Lactate Production by Escherichia coli
journal, November 2010

High Glycolytic Flux Improves Pyruvate Production by a Metabolically Engineered Escherichia coli Strain
journal, September 2008
  • Zhu, Y.; Eiteman, M. A.; Altman, R.
  • Applied and Environmental Microbiology, Vol. 74, Issue 21, p. 6649-6655
  • DOI: 10.1128/AEM.01610-08