Microorganisms and methods for producing pyruvate, ethanol, and other compounds
Abstract
Microorganisms comprising modifications for producing pyruvate, ethanol, and other compounds. The microorganisms comprise modifications that reduce or ablate activity of one or more of pyruvate dehydrogenase, 2-oxoglutarate dehydrogenase, phosphate acetyltransferase, acetate kinase, pyruvate oxidase, lactate dehydrogenase, cytochrome terminal oxidase, succinate dehydrogenase, 6-phosphogluconate dehydrogenase, glutamate dehydrogenase, pyruvate formate lyase, pyruvate formate lyase activating enzyme, and isocitrate lyase. The microorganisms optionally comprise modifications that enhance expression or activity of pyruvate decarboxylase and alcohol dehydrogenase. The microorganisms are optionally evolved in defined media to enhance specific production of one or more compounds. Methods of producing compounds with the microorganisms are provided.
- Inventors:
- Issue Date:
- Research Org.:
- Wisconsin Alumni Research Foundation, Madison, WI (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1568147
- Patent Number(s):
- 10246725
- Application Number:
- 15/815,327
- Assignee:
- Wisconsin Alumni Research Foundation (Madison, WI)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FC02-07ER64494; SC0008103
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 11/16/2017
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Reed, Jennifer L., and Zhang, Xiaolin. Microorganisms and methods for producing pyruvate, ethanol, and other compounds. United States: N. p., 2019.
Web.
Reed, Jennifer L., & Zhang, Xiaolin. Microorganisms and methods for producing pyruvate, ethanol, and other compounds. United States.
Reed, Jennifer L., and Zhang, Xiaolin. Tue .
"Microorganisms and methods for producing pyruvate, ethanol, and other compounds". United States. https://www.osti.gov/servlets/purl/1568147.
@article{osti_1568147,
title = {Microorganisms and methods for producing pyruvate, ethanol, and other compounds},
author = {Reed, Jennifer L. and Zhang, Xiaolin},
abstractNote = {Microorganisms comprising modifications for producing pyruvate, ethanol, and other compounds. The microorganisms comprise modifications that reduce or ablate activity of one or more of pyruvate dehydrogenase, 2-oxoglutarate dehydrogenase, phosphate acetyltransferase, acetate kinase, pyruvate oxidase, lactate dehydrogenase, cytochrome terminal oxidase, succinate dehydrogenase, 6-phosphogluconate dehydrogenase, glutamate dehydrogenase, pyruvate formate lyase, pyruvate formate lyase activating enzyme, and isocitrate lyase. The microorganisms optionally comprise modifications that enhance expression or activity of pyruvate decarboxylase and alcohol dehydrogenase. The microorganisms are optionally evolved in defined media to enhance specific production of one or more compounds. Methods of producing compounds with the microorganisms are provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Apr 02 00:00:00 EDT 2019},
month = {Tue Apr 02 00:00:00 EDT 2019}
}
Works referenced in this record:
Microbial Production of Pyruvate and Pyruvate Derivatives
patent-application, December 2010
- Eiteman, Mark; Altman, Elliot; Zhu, Yihui
- US Patent Application 12/825059; 20100304450