Microorganisms and methods for producing 2-pyrone-4,6-dicarboxylic acid and other compounds
Abstract
Recombinant microorganisms configured for enhanced production of compounds such as 2-pyrone-4,6-dicarboxylic acid (PDC) and methods of using the recombinant microorganisms for the production of these compounds. The recombinant microorganisms include one or more modifications that reduce 2-pyrone-4,6-dicarboxylic acid (PDC) hydrolase activity, 4-carboxy-2-hydroxy-6-methoxy-6-oxohexa-2,4-dienoate (CHMOD) cis-trans isomerase activity, 4-carboxy-2-hydroxy-6-methoxy-6-oxohexa-2,4-dienoate (CHMOD) methyl esterase activity, and/or vanillate/3-O-methylgallate O-demethylase activity. The recombinant microorganisms can be used to generate PDC from media comprising plant-derived phenolics, such as syringyl phenolics, guaiacyl phenolics, and p-hydroxyphenyl phenolics. The plant-derived phenolics can be derived from pretreated lignin, including depolymerized lignin or other chemically altered lignin.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1824050
- Patent Number(s):
- 11028418
- Application Number:
- 16/795,111
- Assignee:
- Wisconsin Alumni Research Foundation (Madison, WI)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FC02-07ER64494; SC0018409
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 02/19/2020
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Noguera, Daniel R., Donohue, Timothy James, Perez, Jose Manuel, Kontur, Wayne S., and Umana Chapeton, German Eduardo. Microorganisms and methods for producing 2-pyrone-4,6-dicarboxylic acid and other compounds. United States: N. p., 2021.
Web.
Noguera, Daniel R., Donohue, Timothy James, Perez, Jose Manuel, Kontur, Wayne S., & Umana Chapeton, German Eduardo. Microorganisms and methods for producing 2-pyrone-4,6-dicarboxylic acid and other compounds. United States.
Noguera, Daniel R., Donohue, Timothy James, Perez, Jose Manuel, Kontur, Wayne S., and Umana Chapeton, German Eduardo. Tue .
"Microorganisms and methods for producing 2-pyrone-4,6-dicarboxylic acid and other compounds". United States. https://www.osti.gov/servlets/purl/1824050.
@article{osti_1824050,
title = {Microorganisms and methods for producing 2-pyrone-4,6-dicarboxylic acid and other compounds},
author = {Noguera, Daniel R. and Donohue, Timothy James and Perez, Jose Manuel and Kontur, Wayne S. and Umana Chapeton, German Eduardo},
abstractNote = {Recombinant microorganisms configured for enhanced production of compounds such as 2-pyrone-4,6-dicarboxylic acid (PDC) and methods of using the recombinant microorganisms for the production of these compounds. The recombinant microorganisms include one or more modifications that reduce 2-pyrone-4,6-dicarboxylic acid (PDC) hydrolase activity, 4-carboxy-2-hydroxy-6-methoxy-6-oxohexa-2,4-dienoate (CHMOD) cis-trans isomerase activity, 4-carboxy-2-hydroxy-6-methoxy-6-oxohexa-2,4-dienoate (CHMOD) methyl esterase activity, and/or vanillate/3-O-methylgallate O-demethylase activity. The recombinant microorganisms can be used to generate PDC from media comprising plant-derived phenolics, such as syringyl phenolics, guaiacyl phenolics, and p-hydroxyphenyl phenolics. The plant-derived phenolics can be derived from pretreated lignin, including depolymerized lignin or other chemically altered lignin.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jun 08 00:00:00 EDT 2021},
month = {Tue Jun 08 00:00:00 EDT 2021}
}
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