Phenolic acid esterases, coding sequences and methods

Blum, David L; Kataeva, Irina; Li, Xin-Liang; Ljungdahl, Lars G

Phenolic acid esterases, coding sequences and methods

Patent · Mon Dec 31 23:00:00 EST 2001

OSTI ID:874323

Blum, David L ^[1]; Kataeva, Irina ^[2]; Li, Xin-Liang ^[2]; Ljungdahl, Lars G ^[2]

San Diego, CA
Athens, GA

Described herein are four phenolic acid esterases, three of which correspond to domains of previously unknown function within bacterial xylanases, from XynY and XynZ of Clostridium thermocellum and from a xylanase of Ruminococcus. The fourth specifically exemplified xylanase is a protein encoded within the genome of Orpinomyces PC-2. The amino acids of these polypeptides and nucleotide sequences encoding them are provided. Recombinant host cells, expression vectors and methods for the recombinant production of phenolic acid esterases are also provided.

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Research Organization:: University of Georgia

DOE Contract Number:: FG05-93ER20127

Assignee:: University of Georgia Research Foundation, Inc. (Athens, GA)

Patent Number(s):: US 6365390

OSTI ID:: 874323

Country of Publication:: United States

Language:: English

References (21)

Evidence for a general role for non-catalytic thermostabilizing domains in xylanases from thermophilic bacteria Fontes, C. M. G. A.; Hazlewood, G. P.; Morag, E. Biochemical Journal, Vol. 307, Issue 1 https://doi.org/10.1042/bj3070151	journal	April 1995
Expression of a Butyrivibrio fibrisolvens E14 gene (cinB) encoding an enzyme with cinnamoyl ester hydrolase activity is negatively regulated by the product of an adjacent gene (cinR) Dalrymple, B. P.; Swadling, Y. Microbiology, Vol. 143, Issue 4 https://doi.org/10.1099/00221287-143-4-1203	journal	April 1997
A cyclophilin from the polycentric anaerobic rumen fungus Orpinomyces sp. strain PC-2 is highly homologous to vertebrate cyclophilin B. Chen, H.; Li, X. L.; Ljungdahl, L. G. Proceedings of the National Academy of Sciences, Vol. 92, Issue 7 https://doi.org/10.1073/pnas.92.7.2587	journal	March 1995
Nucleotide sequence and deletion analysis of the xylanase gene (xynZ) of Clostridium thermocellum. Grépinet, O.; Chebrou, M. C.; Béguin, P. Journal of Bacteriology, Vol. 170, Issue 10, p. 4582-4588 https://doi.org/10.1128/JB.170.10.4582-4588.1988	journal	October 1988
Ferulic Acid Esterase Activity from Schizophyllum commune† MacKenzie, C. Roger; Bilous, Doris Applied and Environmental Microbiology, Vol. 54, Issue 5 https://doi.org/10.1128/AEM.54.5.1170-1173.1988	journal	January 1988
The Cellulosome: The Exocellular Organelle of Clostridium Felix, C. R.; Ljungdahl, L. G. Annual Review of Microbiology, Vol. 47, Issue 1 https://doi.org/10.1146/annurev.mi.47.100193.004043	journal	October 1993
Assay for trans-p-coumaroyl esterase using a specific substrate from plant cell walls Borneman, William S.; Hartley, Roy D.; Himmelsbach, David S. Analytical Biochemistry, Vol. 190, Issue 1 https://doi.org/10.1016/0003-2697(90)90145-Y	journal	October 1990
A bifunctional enzyme, with separate xylanase and beta(1,3-1,4)-glucanase domains, encoded by the xynD gene of Ruminococcus flavefaciens. Flint, H. J.; Martin, J.; McPherson, C. A. Journal of Bacteriology, Vol. 175, Issue 10 https://doi.org/10.1128/JB.175.10.2943-2951.1993	journal	January 1993
A modular esterase from Pseudomonas fluorescens subsp. cellulosa contains a non-catalytic cellulose-binding domain Ferreira, L. M.; Wood, T. M.; Williamson, G. Biochemical Journal, Vol. 294, Issue 2 https://doi.org/10.1042/bj2940349	journal	September 1993
Identification and Characterization of Cellulose-Binding Domains in Xylanase A of Clostridium stercorarium Sakka, Kazuo; Takada, Goro; Karita, Shuichi Annals of the New York Academy of Sciences, Vol. 782, Issue 1 https://doi.org/10.1111/j.1749-6632.1996.tb40565.x	journal	May 1996
The purification and characterization of 4-hydroxy-3-methoxycinnamic (ferulic) acid esterase from Streptomyces olivochromogenes Faulds, C. B.; Williamson, G. Journal of General Microbiology, Vol. 137, Issue 10 https://doi.org/10.1099/00221287-137-10-2339	journal	October 1991
Plant cell wall degrading enzyme complexes from the cellulolytic rumen bacterium Ruminococcus flavefaciens Kirby, Jim; Aurilia, Vincenzo; McCrae, Sheila I. Biochemical Society Transactions, Vol. 26, Issue 2 https://doi.org/10.1042/bst026s169	journal	May 1998
The faeA genes from Aspergillus niger and Aspergillus tubingensis encode ferulic acid esterases involved in degradation of complex cell wall polysaccharides. de Vries, R. P.; Michelsen, B.; Poulsen, C. H. Applied and environmental microbiology, Vol. 63, Issue 12 https://doi.org/10.1128/AEM.63.12.4638-4644.1997	journal	January 1997
Nucleotide Sequence of the Clostridium stercorarium xynA Gene Encoding Xylanase A: Identification of Catalytic and Cellulose Binding Domains Sakka, Kazuo; Kojima, Yuzo; Kondo, Tatsuki Bioscience, Biotechnology, and Biochemistry, Vol. 57, Issue 2 https://doi.org/10.1271/bbb.57.273	journal	January 1993
Lignin-ferulate cross-links in grasses: active incorporation of ferulate polysaccharide esters into ryegrass lignins Ralph, John; Grabber, John H.; Hatfield, Ronald D. Carbohydrate Research, Vol. 275, Issue 1, p. 167-178 https://doi.org/10.1016/0008-6215(95)00237-N	journal	September 1995
Esterases of xylan-degrading microorganisms: Production, properties, and significance Christov, Lyudmil Pavlov; Prior, Bernard Alexander Enzyme and Microbial Technology, Vol. 15, Issue 6 https://doi.org/10.1016/0141-0229(93)90078-G	journal	June 1993
Purification and characterization of a feruloyl/ρ-coumaroyl esterase from solid-state cultures of the aerobic fungus Penicillium pinophilum Castanares, Arantza; Wood, Thomas M. Biochemical Society Transactions, Vol. 20, Issue 3 https://doi.org/10.1042/bst020275s	journal	August 1992
Cloning of a gene encoding cinnamoyl ester hydrolase from the ruminai bacterium Butyrivibrio fibrisolvens E14 by a novel method Dalrymple, Brian P.; Swadling, Yolande; Cybinski, Daisy H. FEMS Microbiology Letters, Vol. 143, Issue 2-3 https://doi.org/10.1111/j.1574-6968.1996.tb08469.x	journal	October 1996
Purification and partial characterization of two feruloyl esterases from the anaerobic fungus Neocallimastix strain MC-2. Borneman, W. S.; Ljungdahl, L. G.; Hartley, R. D. Applied and Environmental Microbiology, Vol. 58, Issue 11 https://doi.org/10.1128/AEM.58.11.3762-3766.1992	journal	January 1992
Esterase Activities of Fibrobacter succinogenes subsp. succinogenes S85 McDermid, Kevin P.; MacKenzie, C. Roger; Forsberg, Cecil W. Applied and Environmental Microbiology, Vol. 56, Issue 1 https://doi.org/10.1128/AEM.56.1.127-132.1990	journal	January 1990
Butyrivibriospp. and Other Xylanolytic Microorganisms From the Rumen have Cinnamoyl Esterase Activity McSweeney, C. S.; Dulieu, A.; Bunch, R. Anaerobe, Vol. 4, Issue 1 https://doi.org/10.1006/anae.1997.0130	journal	February 1998

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/435/530/999/
acid
acid ester
acids
amino
amino acid
bacterial
cells
clostridium
coding
coding sequence
described
domains
encoded
encoding
esterases
exemplified
expression
fourth
function
genome
host
methods
nucleotide
orpinomyces
pc-2
phenolic
polypeptides
previously
production
protein
provided
recombinant
recombinant host
ruminococcus
sequences
specifically
thermocellum
vectors
xylanase
xylanases
xyny
xynz

Phenolic acid esterases, coding sequences and methods

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References (21)

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