Methods of sequencing and detection using energy transfer labels with cyanine dyes as donor chromophores
Patent
·
OSTI ID:873390
- Orinda, CA
- Moraga, CA
- Richmond, CA
- Redwood City, CA
Cyanine dyes are used as the donor fluorophore in energy transfer labels in which light energy is absorbed by a donor fluorophore and transferred to an acceptor fluorophore which responds to the transfer by emitting fluorescent light for detection. The cyanine dyes impart an unusually high sensitivity to the labels thereby improving their usefulness in a wide variety of biochemical procedures, particularly nucleic acid sequencing, nucleic acid fragment sizing, and related procedures.
- Research Organization:
- Univ. of California (United States)
- DOE Contract Number:
- FG03-91ER61125
- Assignee:
- Regents of University of California (Oakland, CA)
- Patent Number(s):
- US 6150107
- OSTI ID:
- 873390
- Country of Publication:
- United States
- Language:
- English
Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.
|
journal | June 1995 |
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Related Subjects
methods
sequencing
detection
energy
transfer
labels
cyanine
dyes
donor
chromophores
fluorophore
light
absorbed
transferred
acceptor
responds
emitting
fluorescent
impart
unusually
sensitivity
improving
usefulness
wide
variety
biochemical
procedures
particularly
nucleic
acid
fragment
sizing
related
cyanine dyes
light energy
fluorescent light
energy transfer
nucleic acid
wide variety
acid sequencing
transfer labels
donor fluorophore
fragment sizing
chemical procedures
chemical procedure
acid fragment
donor chromophores
/435/999/
sequencing
detection
energy
transfer
labels
cyanine
dyes
donor
chromophores
fluorophore
light
absorbed
transferred
acceptor
responds
emitting
fluorescent
impart
unusually
sensitivity
improving
usefulness
wide
variety
biochemical
procedures
particularly
nucleic
acid
fragment
sizing
related
cyanine dyes
light energy
fluorescent light
energy transfer
nucleic acid
wide variety
acid sequencing
transfer labels
donor fluorophore
fragment sizing
chemical procedures
chemical procedure
acid fragment
donor chromophores
/435/999/