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Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices

Patent ·
OSTI ID:873161
 [1];  [2];  [3]
  1. Charleston, SC
  2. Mt. Pleasant, SC
  3. Richland, WA
+ Show Author Affiliations
A gelling cell culture medium useful for forming a three dimensional matrix for cell culture in vitro is prepared by copolymerizing an acrylamide derivative with a hydrophilic comonomer to form a reversible (preferably thermally reversible) gelling linear random copolymer in the form of a plurality of linear chains having a plurality of molecular weights greater than or equal to a minimum gelling molecular weight cutoff, mixing the copolymer with an aqueous solvent to form a reversible gelling solution and adding a cell culture medium to the gelling solution to form the gelling cell culture medium. Cells such as chondrocytes or hepatocytes are added to the culture medium to form a seeded culture medium, and temperature of the medium is raised to gel the seeded culture medium and form a three dimensional matrix containing the cells. After propagating the cells in the matrix, the cells may be recovered by lowering the temperature to dissolve the matrix and centrifuging.
Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA
DOE Contract Number:
AC06-76RL01830
Assignee:
Battelle Memorial Institute (Richland, WA); Medical University of South Carolina (Charleston, SC)
Patent Number(s):
US 6103528
OSTI ID:
873161
Country of Publication:
United States
Language:
English

References (6)

Thermally reversible polymer gels for biohybrid artificial pancreas journal May 1996
Fibroblast growth factor stimulates colony formation of differentiated chondrocytes in soft agar journal December 1987
Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels journal August 1982
Treatment of Deep Cartilage Defects in the Knee with Autologous Chondrocyte Transplantation journal October 1994
Develoapmet of a Tissue Analog for Cartilage Repair journal January 1991
Enhanced Tumor Growth of Both Primary and Established Human and Murine Tumor Cells in Athymic Mice After Coinjection With Matrigel journal June 1991

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Related Subjects

/435/424/
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