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Title: Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices

Abstract

A gelling cell culture medium useful for forming a three dimensional matrix for cell culture in vitro is prepared by copolymerizing an acrylamide derivative with a hydrophilic comonomer to form a reversible (preferably thermally reversible) gelling linear random copolymer in the form of a plurality of linear chains having a plurality of molecular weights greater than or equal to a minimum gelling molecular weight cutoff, mixing the copolymer with an aqueous solvent to form a reversible gelling solution and adding a cell culture medium to the gelling solution to form the gelling cell culture medium. Cells such as chondrocytes or hepatocytes are added to the culture medium to form a seeded culture medium, and temperature of the medium is raised to gel the seeded culture medium and form a three dimensional matrix containing the cells. After propagating the cells in the matrix, the cells may be recovered by lowering the temperature to dissolve the matrix and centrifuging.

Inventors:
 [1];  [2];  [3]
  1. (Charleston, SC)
  2. (Mt. Pleasant, SC)
  3. (Richland, WA)
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA
OSTI Identifier:
873161
Patent Number(s):
US 6103528
Assignee:
Battelle Memorial Institute (Richland, WA); Medical University of South Carolina (Charleston, SC) PNNL
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
reversible; gelling; culture; media; in-vitro; cell; three-dimensional; matrices; medium; useful; forming; dimensional; matrix; vitro; prepared; copolymerizing; acrylamide; derivative; hydrophilic; comonomer; form; preferably; thermally; linear; random; copolymer; plurality; chains; molecular; weights; equal; minimum; weight; cutoff; mixing; aqueous; solvent; solution; adding; cells; chondrocytes; hepatocytes; added; seeded; temperature; raised; containing; propagating; recovered; lowering; dissolve; centrifuging; reversible gelling; random copolymer; aqueous solvent; culture medium; molecular weight; cell culture; molecular weights; thermally reversible; dimensional matrix; matrix containing; linear random; vitro cell; weight cu; acrylamide derivative; /435/424/

Citation Formats

An, Yuehuei H., Mironov, Vladimir A., and Gutowska, Anna. Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices. United States: N. p., 2000. Web.
An, Yuehuei H., Mironov, Vladimir A., & Gutowska, Anna. Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices. United States.
An, Yuehuei H., Mironov, Vladimir A., and Gutowska, Anna. Sat . "Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices". United States. https://www.osti.gov/servlets/purl/873161.
@article{osti_873161,
title = {Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices},
author = {An, Yuehuei H. and Mironov, Vladimir A. and Gutowska, Anna},
abstractNote = {A gelling cell culture medium useful for forming a three dimensional matrix for cell culture in vitro is prepared by copolymerizing an acrylamide derivative with a hydrophilic comonomer to form a reversible (preferably thermally reversible) gelling linear random copolymer in the form of a plurality of linear chains having a plurality of molecular weights greater than or equal to a minimum gelling molecular weight cutoff, mixing the copolymer with an aqueous solvent to form a reversible gelling solution and adding a cell culture medium to the gelling solution to form the gelling cell culture medium. Cells such as chondrocytes or hepatocytes are added to the culture medium to form a seeded culture medium, and temperature of the medium is raised to gel the seeded culture medium and form a three dimensional matrix containing the cells. After propagating the cells in the matrix, the cells may be recovered by lowering the temperature to dissolve the matrix and centrifuging.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2000},
month = {Sat Jan 01 00:00:00 EST 2000}
}

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