Method for performing site-specific affinity fractionation for use in DNA sequencing

Patent · Thu Dec 31 23:00:00 EST 1998

OSTI ID:872290

Mirzabekov, Andrei Darievich ^[1]; Lysov, Yuri Petrovich ^[1]; Dubley, Svetlana A ^[1]

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1. Moscow, RU

A method for fractionating and sequencing DNA via affinity interaction is provided comprising contacting cleaved DNA to a first array of oligonucleotide molecules to facilitate hybridization between said cleaved DNA and the molecules; extracting the hybridized DNA from the molecules; contacting said extracted hybridized DNA with a second array of oligonucleotide molecules, wherein the oligonucleotide molecules in the second array have specified base sequences that are complementary to said extracted hybridized DNA; and attaching labeled DNA to the second array of oligonucleotide molecules, wherein the labeled re-hybridized DNA have sequences that are complementary to the oligomers. The invention further provides a method for performing multi-step conversions of the chemical structure of compounds comprising supplying an array of polyacrylamide vessels separated by hydrophobic surfaces; immobilizing a plurality of reactants, such as enzymes, in the vessels so that each vessel contains one reactant; contacting the compounds to each of the vessels in a predetermined sequence and for a sufficient time to convert the compounds to a desired state; and isolating the converted compounds from said array.

• Research Organization: Argonne National Laboratory (ANL), Argonne, IL
• DOE Contract Number: W-31109-ENG-38
• Assignee: University of Chicago (Chicago, IL)
• Patent Number(s): US 5905024
• OSTI ID: 872290
• Country of Publication: United States
• Language: English

Related Subjects

/435/427/436/999/
affinity
affinity fractionation
affinity interaction
array
attaching
base
base sequence
base sequences
chemical
cleaved
cleaved dna
complementary
compounds
compounds comprising
comprising
comprising contacting
comprising supplying
contacting
contacting cleaved
contains
conversions
convert
converted
desired
dna
dna sequencing
dna via
enzymes
extracted
extracting
facilitate
fractionating
fractionation
hybridization
hybridized
hybridized dna
hydrophobic
immobilizing
interaction
isolating
labeled
labeled dna
method
molecules
multi-step
oligomers
oligonucleotide
oligonucleotide molecules
performing
performing site-specific
plurality
polyacrylamide
predetermined
predetermined sequence
provided
provided comprising
provides
re-hybridized
reactant
reactants
separated
sequence
sequences
sequencing
sequencing dna
site-specific
specific affinity
specified
structure
sufficient
sufficient time
supplying
surfaces
time
vessel
vessel contains
vessels
via
via affinity