Miniaturized reaction vessel system, method for performing site-specific biochemical reactions and affinity fractionation for use in DNA sequencing
- Moscow, RU
A method for fractionating and sequencing DNA via affinity interaction is provided comprising contacting cleaved DNA to a first array of oligonucleotide molecules to facilitate hybridization between said cleaved DNA and the molecules; extracting the hybridized DNA from the molecules; contacting said extracted hybridized DNA with a second array of oligonucleotide molecules, wherein the oligonucleotide molecules in the second array have specified base sequences that are complementary to said extracted hybridized DNA; and attaching labeled DNA to the second array of oligonucleotide molecules, wherein the labeled re-hybridized DNA have sequences that are complementary to the oligomers. The invention further provides a method for performing multi-step conversions of the chemical structure of compounds comprising supplying an array of polyacrylamide vessels separated by hydrophobic surfaces; immobilizing a plurality of reactants, such as enzymes, in the vessels so that each vessel contains one reactant; contacting the compounds to each of the vessels in a predetermined sequence and for a sufficient time to convert the compounds to a desired state; and isolating the converted compounds from said array.
- Research Organization:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- DOE Contract Number:
- W-31109-ENG-38
- Assignee:
- University of Chicago (Chicago, IL)
- Patent Number(s):
- US 6143499
- OSTI ID:
- 873359
- Country of Publication:
- United States
- Language:
- English
Ligation of restriction endonuclease-generated DNA fragments using immobilized T4 DNA ligase
|
journal | July 1982 |
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reaction
vessel
method
performing
site-specific
biochemical
reactions
affinity
fractionation
dna
sequencing
fractionating
via
interaction
provided
comprising
contacting
cleaved
array
oligonucleotide
molecules
facilitate
hybridization
extracting
hybridized
extracted
specified
base
sequences
complementary
attaching
labeled
re-hybridized
oligomers
provides
multi-step
conversions
chemical
structure
compounds
supplying
polyacrylamide
vessels
separated
hydrophobic
surfaces
immobilizing
plurality
reactants
enzymes
contains
reactant
predetermined
sequence
sufficient
time
convert
desired
isolating
converted
predetermined sequence
comprising supplying
hybridized dna
cleaved dna
compounds comprising
sequencing dna
base sequences
labeled dna
vessel contains
provided comprising
chemical reaction
reaction vessel
dna sequencing
sufficient time
oligonucleotide molecules
chemical reactions
base sequence
comprising contacting
biochemical reactions
performing site-specific
via affinity
affinity fractionation
affinity interaction
dna via
contacting cleaved
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