Method for performing site-specific affinity fractionation for use in DNA sequencing

Patent · Tue May 18 04:00:00 EDT 1999

OSTI ID:6281441

Mirzabekov, A D; Lysov, Y P; Dubley, S A

A method for fractionating and sequencing DNA via affinity interaction is provided comprising contacting cleaved DNA to a first array of oligonucleotide molecules to facilitate hybridization between the cleaved DNA and the molecules; extracting the hybridized DNA from the molecules; contacting the extracted hybridized DNA with a second array of oligonucleotide molecules, wherein the oligonucleotide molecules in the second array have specified base sequences that are complementary to the extracted hybridized DNA; and attaching labeled DNA to the second array of oligonucleotide molecules, wherein the labeled re-hybridized DNA have sequences that are complementary to the oligomers. The invention further provides a method for performing multi-step conversions of the chemical structure of compounds comprising supplying an array of polyacrylamide vessels separated by hydrophobic surfaces; immobilizing a plurality of reactants, such as enzymes, in the vessels so that each vessel contains one reactant; contacting the compounds to each of the vessels in a predetermined sequence and for a sufficient time to convert the compounds to a desired state; and isolating the converted compounds from the array. 14 figs.

• Sponsoring Organization: DOE; USDOE, Washington, DC (United States)
• DOE Contract Number: W-31109-ENG-38
• Assignee: Univ. of Chicago, IL (United States)
• Patent Number(s): A; US 5905024
• Application Number: PPN: US 8-768893
• OSTI ID: 6281441
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
BIOTECHNOLOGY
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
GENETIC ENGINEERING
HYBRIDIZATION
MOLECULAR STRUCTURE
NUCLEIC ACID HYBRIDIZATION
NUCLEIC ACIDS
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
STRUCTURAL CHEMICAL ANALYSIS