Method for detection of polymorphic restriction sites and nucleic acid sequences
A method is detected for detecting the presence or absence of at least one specific restriction site in a specific nucleic acid sequence comprising the steps of: (a) hybridizing the nucleic acid sequence in solution with an oligonucleotide probe for each restriction site detected. A probe is complementary to a region in the nucleic acid sequence spanning the respective restriction site of the probe is labeled at the end which is nearer to the respective restriction site than the other end of the probe; (b) digesting the hybridized nucleic acid sequence with a restriction endonuclease for each restriction site detected by each probe capable of cleaving its respective probe at the restriction site being detected. This produces labeled and unlabeled oligomer fragments.; (c) separating any labeled cleaved oligomer fragments from labeled uncleaved oligomers, and (d) detecting the presence or absence of labeled oligomer fragments.
- Assignee:
- Cetus Corp., Emeryville, CA
- Patent Number(s):
- US 4683194
- OSTI ID:
- 6076565
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CLEAVAGE
CRYSTAL STRUCTURE
DESIGN
DETECTION
DNA SEQUENCING
DNA-ASE
ENDONUCLEASES
ENZYMES
ESTERASES
HYBRIDIZATION
HYDROLASES
LABELLING
MICROSTRUCTURE
NUCLEIC ACIDS
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PROBES
STRUCTURAL CHEMICAL ANALYSIS