Method for detection of polymorphic restriction sites and nucleic acid sequences

Patent · Tue Jul 28 00:00:00 EDT 1987

OSTI ID:6076565

Saiki, R K; Erlich, H A

A method is detected for detecting the presence or absence of at least one specific restriction site in a specific nucleic acid sequence comprising the steps of: (a) hybridizing the nucleic acid sequence in solution with an oligonucleotide probe for each restriction site detected. A probe is complementary to a region in the nucleic acid sequence spanning the respective restriction site of the probe is labeled at the end which is nearer to the respective restriction site than the other end of the probe; (b) digesting the hybridized nucleic acid sequence with a restriction endonuclease for each restriction site detected by each probe capable of cleaving its respective probe at the restriction site being detected. This produces labeled and unlabeled oligomer fragments.; (c) separating any labeled cleaved oligomer fragments from labeled uncleaved oligomers, and (d) detecting the presence or absence of labeled oligomer fragments.

• Assignee: Cetus Corp., Emeryville, CA
• Patent Number(s): US 4683194
• OSTI ID: 6076565
• Resource Relation: Patent File Date: Filed date 28 Mar 1985
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA SEQUENCING
ENDONUCLEASES
PROBES
DESIGN
CLEAVAGE
DETECTION
HYBRIDIZATION
LABELLING
OLIGONUCLEOTIDES
CRYSTAL STRUCTURE
DNA-ASE
ENZYMES
ESTERASES
HYDROLASES
MICROSTRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
STRUCTURAL CHEMICAL ANALYSIS
550200* - Biochemistry
550400 - Genetics