Methods for detecting nucleic acid sequences
Patent
·
OSTI ID:5026872
This patent describes a method for detecting a single-stranded target nucleic acid. It comprises: obtaining the single-stranded target nucleic acid; forming a reaction mixture which includes the target nucleic acid and a complementary single-stranded nucleic acid probe under conditions which allow the target nucleic acid and the probe to hybridize to each other and form a double-stranded, target-probe complex, the probe being present in molar excess relative to the target; treating the double-stranded, target-probe complex so as to cleave the probe within a predetermined sequence of the scissile nucleic acid linkage and thereby form at least one intact DNA-containing oligonucleotide fragment from the probe, such fragment being, or being treated so as to be, no longer capable of remaining hybridized to the target nucleic acid; detecting the intact DNA-containing fragments so formed and thereby detecting the single-stranded target nucleic acid.
- Assignee:
- Meiogenics U.S. Ltd. Partnership, Columbia, MD (USA)
- Patent Number(s):
- A; US 5011769
- Application Number:
- PPN: US 7-187814
- OSTI ID:
- 5026872
- Country of Publication:
- United States
- Language:
- English
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