DNA sequencing with pyrophosphatase

Patent · Sun Dec 31 23:00:00 EST 1995

OSTI ID:870336

Tabor, Stanley ^[1]; Richardson, Charles C ^[2]

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1. Cambridge, MA
2. Chestnut Hill, MA

A kit or solution for use in extension of an oligonucleotide primer having a first single-stranded region on a template molecule having a second single-stranded region homologous to the first single-stranded region, comprising a first agent able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mixture, and a second agent able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.

• Research Organization: Harvard University
• DOE Contract Number: FG02-88ER60688
• Assignee: President and Fellows of Harvard College (Cambridge, MA)
• Patent Number(s): US 5498523
• Application Number: 08/275,339
• OSTI ID: 870336
• Country of Publication: United States
• Language: English

Related Subjects

/435/436/
absence
agent
amount
below
comprising
dna
dna sequencing
extension
homologous
kit
mixture
molecule
oligonucleotide
oligonucleotide primer
primer
produced
pyrophosphatase
pyrophosphate
reaction
reaction mixture
reduce
region
sequencing
single-stranded
single-stranded region
solution
template
template molecule