DNA sequencing
A method for sequencing a strand of DNA. It comprises providing the strand hybridized with a primer able to hybridize to the strand, to give an hybridized mixture, incubating the hybridized mixture with four deoxyribonucleoside triphosphates, a DNA polymerase, and a first chain terminating agent, wherein the DNA polymerase causes the primer to be elongated to form a first series of first DNA products differing in the length of the elongated primer, each the first DNA product having a the chain terminating agent at its elongated end. The number of molecules of each the first DNA products is approximately the same for substantially all DNA products differing in length by no more than 20 bases, and providing a second chain terminating agent in the hybridized mixture at a concentration different from the first chain terminating agent.
- Assignee:
- Harvard College, Cambridge, MA (USA)
- Patent Number(s):
- US 7962020; A
- Application Number:
- PPN: US s 7-218103
- OSTI ID:
- 6522147
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
DNA POLYMERASES
DNA SEQUENCING
ENZYMES
GENE RECOMBINATION PROTEINS
KINETICS
NUCLEOPROTEINS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PROTEINS
REACTION KINETICS
STRUCTURAL CHEMICAL ANALYSIS
TECHNOLOGY ASSESSMENT
TRANSFERASES