T7 DNA polymerase
A method is described for determining the nucleotide base sequence of a DNA molecule, comprising: annealing the DNA molecule with a primer molecule able to hybridize to the DNA molecule; incubating separate portions of the annealed mixture in at least four vessels, each vessel containing four different deoxynucleoside triphosphates, a processive T7-type DNA polymerase, wherein the polymerase remains bound to the DNA molecule for at least 500 bases before dissociating in an environmental condition used in the extension reaction of a DNA sequencing reaction, separating the DNA products of each incubating reaction according to their size, whereby at least a part of the nucleotide base sequence of the DNA molecule can be determined.
- Assignee:
- President and Fellows of Harvard College, Cambridge, MA
- Patent Number(s):
- US 4795699
- OSTI ID:
- 6465879
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ACTIVATION ENERGY
ANNEALING
CHEMICAL BONDS
DNA
DNA POLYMERASES
DNA SEQUENCING
ENERGY
ENZYMES
HEAT TREATMENTS
HYBRIDIZATION
INCUBATION
MOLECULES
NUCLEIC ACIDS
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES