Dna Sequencing

Tabor, Stanley; Richardson, Charles C

Dna Sequencing

Patent · Tue Apr 25 00:00:00 EDT 1995

OSTI ID:879187

Tabor, Stanley ^[1]; Richardson, Charles C ^[2]

Cambridge, MA
Chestnut Hill, MA

A method for sequencing a strand of DNA, including the steps off: providing the strand of DNA; annealing the strand with a primer able to hybridize to the strand to give an annealed mixture; incubating the mixture with four deoxyribonucleoside triphosphates, a DNA polymerase, and at least three deoxyribonucleoside triphosphates in different amounts, under conditions in favoring primer extension to form nucleic acid fragments complementory to the DNA to be sequenced; labelling the nucleic and fragments; separating them and determining the position of the deoxyribonucleoside triphosphates by differences in the intensity of the labels, thereby to determine the DNA sequence.

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Research Organization:: Harvard University

DOE Contract Number:: FG02-88ER60688

Assignee:: President and Fellows of Harvard College (Cambridge, MA)

Patent Number(s):: US 5409811

Application Number:: 07/869520

OSTI ID:: 879187

Country of Publication:: United States

Language:: English

References (61)

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