DNA sequencing with pyrophosphatase
Patent
·
OSTI ID:203883
A kit or solution is disclosed for use in extension of an oligonucleotide primer having a first single-stranded region on a template molecule and having a second single-stranded region homologous to the first single-stranded region. The first agent is able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mixture. The second agent is able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.
- Research Organization:
- Harvard University
- DOE Contract Number:
- FG02-88ER60688
- Assignee:
- Harvard Coll., Cambridge, MA (United States)
- Patent Number(s):
- US 5,498,523/A/
- Application Number:
- PAN: 8-275,339; CNN: Grant A1-06045
- OSTI ID:
- 203883
- Country of Publication:
- United States
- Language:
- English
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