Apparatus for continuously referenced analysis of reactive components in solution

Patent · Thu Jan 01 04:00:00 EST 1981

OSTI ID:863866

Bostick, William D ^[1]; Denton, Mark S ^[1]; Dinsmore, Stanley R ^[2]

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1. Oak Ridge, TN
2. Norris, TN

A continuously referenced apparatus for measuring the concentration of a reactive chemical species in solution comprises in combination conduit means for introducing a sample solution, means for introducing one or more reactants into a sample solution, a reaction zone in fluid communication with said conduit means wherein a first chemical reaction occurs between said species and reactants, and a stream separator disposed within the conduit means for separating the sample solution into a sample stream and a reference stream. An enzymatic reactor is disposed in fluid communication with only the sample stream wherein a second reaction takes place between the said reactants, species, and reactor enzymes causing the consumption or production of an indicator species in just the sample stream. Measurement means such as a photometric system are disposed in communication with the sample and reference streams, and the outputs of the measurement means are compared to provide a blanked measurement of the concentration of indicator species. A peristaltic pump is provided to equalize flow through the apparatus by evacuation. The apparatus is particularly suitable for measurement of isoenzymes in body tissues or fluids.

• Research Organization: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN
• Assignee: United States of America as represented by United States (Washington, DC)
• Patent Number(s): US 4263406
• OSTI ID: 863866
• Country of Publication: United States
• Language: English

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active chemical
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chemical specie
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combination
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components
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conduit means
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reactive
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reactive components
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sample
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sample stream
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