Method and apparatus for continuously referenced analysis of reactive components in solution
A continuously referenced apparatus for measuring the concentration of a reactive chemical species in solution comprises in combination conduit means for introducing a sample solution, means for introducing one or more reactants into a sample solution, and a stream separator disposed within the conduit means for separating the sample solution into a first sample stream and a second sample stream. A reactor is disposed in fluid communication with the first sample stream. A reaction takes place between the reactants introduced and the reactive chemical species of interest, causing the consumption or production of an indicator species in the first sample stream. Measurement means such as a photometric system are disposed in communication with the first and second sample streams, and the outputs of the measurement means are compared to provide a blanked measurement of the concentration of indicator species. The apparatus is particularly suitable for measurement of isoenzymes in body tissues or fluids.
- DOE Contract Number:
- W-7405-ENG-26
- Assignee:
- Dept. of Energy
- OSTI ID:
- 5077176
- Country of Publication:
- United States
- Language:
- English
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ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
47 OTHER INSTRUMENTATION
BODY FLUIDS
QUANTITATIVE CHEMICAL ANALYSIS
MEASURING INSTRUMENTS
TISSUES
DESIGN
ION EXCHANGE CHROMATOGRAPHY
PROTEINS
BIOLOGICAL MATERIALS
BODY
CHEMICAL ANALYSIS
CHROMATOGRAPHY
MATERIALS
ORGANIC COMPOUNDS
SEPARATION PROCESSES
400104* - Spectral Procedures- (-1987)
550200 - Biochemistry
440300 - Miscellaneous Instruments- (-1989)