Apparatus for continuously referenced analysis of reactive components in solution

Patent · Tue Apr 21 04:00:00 EST 1981

OSTI ID:7145035

Bostick, W D; Denton, M S; Dinsmore, S R

A continuously referenced apparatus for measuring the concentration of a reactive chemical species in solution comprises in combination conduit means for introducing a sample solution, means for introducing one or more reactants into a sample solution, a reaction zone in fluid communication with said conduit means wherein a first chemical reaction occurs between said species and reactants, and a stream separator disposed within the conduit means for separating the sample solution into a sample stream and a reference stream. An enzymatic reactor is disposed in fluid communication with only the sample stream wherein a second reaction takes place between the said reactants, species, and reactor enzymes causing the consumption or production of an indicator species in just the sample stream. Measurement means such as a photometric system are disposed in communication with the sample and reference streams, and the outputs of the measurement means are compared to provide a blanked measurement of the concentration of indicator species. A peristaltic pump is provided to equalize flow through the apparatus by evacuation. The apparatus is particularly suitable for measurement of isoenzymes in body tissues or fluids. 5 figs.

• Assignee: Dept. of Energy, Washington, DC (United States)
• Patent Number(s): US 4263406; A
• Application Number: PPN: US 6-062375
• OSTI ID: 7145035
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CALIBRATION STANDARDS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMATIC HYDROLYSIS
HYDROLYSIS
INDICATORS
ISOENZYMES
LYSIS
MATERIALS
MEASURING INSTRUMENTS
PHOTOMETRY
SAMPLE HOLDERS
SOLVOLYSIS
STANDARDS
TISSUES