Cloning of rat homeobox genes

Journal Article · Sat Oct 01 04:00:00 EDT 1994 · Biochemical Genetics

DOI:https://doi.org/10.1007/BF02426897· OSTI ID:83458

Sakoyama, Yasuhiko; Mizuta, Ikuko; Ogasawara, Naotake ^[1]

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1. Osaka Univ., Suita-shi (Japan); and others

We report the isolation of nine rat cognates of mouse homeoboxes within the four Hox gene clusters and a rat homologue of mouse IPF1 homeobox, RHbox No. 13A. The sequences of nine cloned homeoboxes are highly similar to those of the mouse and human homeoboxes in the Hox clusters. The restriction enzyme sites and map distances between each of the homeoboxes on the rat genome are nearly identical to those of mouse and human. Thus, we conclude that the isolated homeoboxes are the rat homologues of mouse homeoboxes within the four Hox clusters. A novel homeobox RHbox No. 13A is different from the Drosophila Antennapedia (Antp) sequence but is highly similar to the XlHbox8 (Xenopus laevis) and HtrA2 (Helobdella triserialis) homeoboxes. Forty-two amino acids of the last two-thirds of the RHbox No. 13A, XlHbox8, and mouse IPF1 homeodomains completely matched. In addition, these four homeodomains contain a unique His residue in the recognition helix of a helix-turn-helix DNA-binding motif. This His residue is not found in any of the previously published mammalian homeodomain sequences except mouse IPF1. 24 refs., 4 figs.

• Sponsoring Organization: USDOE
• OSTI ID: 83458
• Journal Information: Biochemical Genetics, Journal Name: Biochemical Genetics Journal Issue: 9-10 Vol. 32; ISSN 0006-2928; ISSN BIGEBA
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL EVOLUTION
CHROMOSOMES
DNA SEQUENCING
DNA-CLONING
GENES
GENETIC MAPPING
MICE
NUCLEOTIDES
RATS
TRANSCRIPTION
TRANSCRIPTION FACTORS