Molecular cloning of the human homeobox gene goosecoid (GSC) and mapping of the gene to human chromosome 14q32. 1

Journal Article · Sun May 15 04:00:00 EDT 1994 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1994.1281· OSTI ID:6872520

Blum, M; De Robertis, E M; Geissert, D ^[1]; Kojis, T; Heinzmann, C; Klisak, I; Sparkes, R S ^[2]

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1. Univ. of California, Los Angeles, CA (United States)
2. Univ. of California School of Medicine, Los Angeles, CA (United States)

Goosecoid is a homeobox gene first isolated from a Xenopus dorsal lip cDNA library. Homologous genes have been isolated from mouse, zebrafish, and chick. In all species examined, the gene is expressed and plays an important role during the process of gastrulation in early embryonic development. The authors report here the cloning of the human goosecoid (GSC) from a genomic library and the sequence of its encoded protein. The genomic organization and protein sequence of the human gene are highly conserved with respect to those of its Xenopus and mouse counterparts: all three genes consist of three exons, with conserved exon-intron boundaries. The sequence of the homeo-domain is 100% conserved in most vertebrates. Using somatic cell hybrid and chromosomal in situ hybridization, the gene was mapped to chromosome 14q32.1. 30 refs., 3 figs., 2 tabs.

• OSTI ID: 6872520
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 21:2; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
CHROMOSOMES
CLONING
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GENE REGULATION
GENETIC MAPPING
HUMAN CHROMOSOME 14
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
MOLECULAR STRUCTURE
ONTOGENESIS
STRUCTURAL CHEMICAL ANALYSIS