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Cloning of fragments of novel homeobox genes expressed during regeneration in planarians

Journal Article · · Russian Journal of Developmental Biology
OSTI ID:83471
The polymerase chain reaction with degenerate primers corresponding to the most conservative amino acids 16-21 (ELEKEF) and 49-54 (WPQNRR) of the Antennapedia class homeodomains was used for the amplification of cDNA from regenerating planarians (asexual race of Dugesia tigrina). A total of six new Antennapedia-like homeobox sequences, designated Dutarh-1-Dutarh-6 (Dugesia tigrina asexual race homeobox gene), have been identified. Their comparison with other homeobox genes using a Genebee software (the EMBL Data Library) showed that all sequences except Dutarh-6 belong to the Antennapedia class. Dutarh-6 is closely related to a recently described novel homeobox gene subfamily which includes mouse mesodermal homeobox genes Max-1 and Max-2 and rat homeobox gene Gax. 17 refs., 2 figs.
OSTI ID:
83471
Journal Information:
Russian Journal of Developmental Biology, Journal Name: Russian Journal of Developmental Biology Journal Issue: 6 Vol. 25; ISSN RJDBE2; ISSN 1062-3604
Country of Publication:
United States
Language:
English

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