Molecular cloning and localization of the human GAX gene to 7p21

Journal Article · Thu Dec 01 04:00:00 EST 1994 · Genomics

DOI:https://doi.org/10.1006/geno.1994.1663· OSTI ID:241039

LePage, D F ^[1]; Altomare, D A; Testa, J R ^[2]

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1. Case Western Reserve Univ., Cleveland, OH (United States)
2. Fox Chase Cancer Center, Philadelphia, PA (United States); and others

The GAX homeobox gene is expressed in the cardiovascular tissues of the adult rat, including heart, lung, kidney, and blood vessels. In the vasculature it is specifically expressed in quiescent smooth muscle cells, but its expression is rapidly down-regulated when these cells are stimulated to proliferate with mitogens. Since vascular smooth muscle cell proliferation is important in the pathology of blood vessel disorders, the human GAX gene was isolated and characterized. The human GAX cDNA was obtained by an anchored-PCR approach using cDNA templates from cardiovascular tissues and amplification primers designed from sequence information of the rat GAK cDNA and the homeodomain-containing exon of the human GAX gene. The human and rat GAX gene coding sequences are 98% conserved at the amino acid level and 83% conserved at the nucleotide level. Similar to rat, the human homolog contains a CAX trinucleotide repeat N-terminal to the homeodomain that encodes for a stretch of 17 consecutive histidine or glutamine residues. The human GAX locus was mapped by fluorescence in situ hybridization to the short arm of chromosome 7 at band p21. The human cDNA sequence will be useful for analyses of GAX gene expression in cardiovascular diseases. 30 refs., 3 figs., 1 tab.

• OSTI ID: 241039
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 24; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AMINO ACIDS
CELL PROLIFERATION
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
EXONS
FLUORESCENCE
GENE REGULATION
GENES
GENETIC MAPPING
HEMIC DISEASES
HUMAN CHROMOSOME 7
NUCLEOTIDES
POLYMERASE CHAIN REACTION
TRANSCRIPTION