Fused quartz substrates for microchip electrophoresis

Journal Article · Sat Jul 01 04:00:00 EDT 1995 · Analytical Chemistry (Washington)

DOI:https://doi.org/10.1021/ac00109a026· OSTI ID:81354

Jacobson, S C; Moore, A W; Ramsey, J M ^[1]

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1. Oak Ridge National Lab., TN (United States)

A fused quartz microchip is fabricated to perform capillary electrophoresis of metal ions complexed with 8-hydroxyquinoline-5-sulfonic acid (HQS). The channel manifold on the quartz substrate is fabricated using standard photolithographic, etching, and deposition techniques. By incorporating a direct bonding technique during the fabrication of the microchip, the substrate and cover plate can be fused together below the melting temperature for fused quartz. To enhance the resolution for the separation, the electroosmotic flow is minimized by covalently bonding polyacrylamide to the channel walls. A separation length of 16.5 mm and separation field strength of 870 V/cm enable separations to be performed in {<=}15 s. By increasing the concentration of HQS from 5 mM to 20 mM, the separation efficiency improves by approximately 3 times. The low background signal from the fused quartz substrate results in mass detection limits of 85, 61, and 134 amol and concentration detection limits of 46, 57, and 30 ppb for Zn, Cd, and Al, respectively. 30 refs., 6 figs., 2 tabs.

• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 81354
• Journal Information: Analytical Chemistry (Washington), Journal Name: Analytical Chemistry (Washington) Journal Issue: 13 Vol. 67; ISSN ANCHAM; ISSN 0003-2700
• Country of Publication: United States
• Language: English

Related Subjects

36 MATERIALS SCIENCE
40 CHEMISTRY
42 ENGINEERING
44 INSTRUMENTATION
INCLUDING NUCLEAR AND PARTICLE DETECTORS
ALUMINIUM
CADMIUM
CAPILLARIES
DETECTION
ELECTROPHORESIS
FABRICATION
MICROANALYSIS
MICROPROCESSORS
QUALITATIVE CHEMICAL ANALYSIS
QUANTITATIVE CHEMICAL ANALYSIS
QUARTZ
SUBSTRATES
SULFONIC ACIDS
ZINC