Effects of injection schemes and column geometry on the performance of microchip electrophoresis devices

Journal Article · Fri Apr 01 04:00:00 EST 1994 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00079a028· OSTI ID:7166277

Jacobson, S C; Hergenroeder, R; Koutny, L B; Warmack, R J; Ramsey, J M ^[1]

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1. Oak Ridge National Lab., TN (United States)

A glass microchip column was fabricated for free-solution electrophoresis. The channels were wet chemically etched on a substrate using standard photolithographic techniques and were sealed using a direct bonding technique. Two methods of sample introduction are evaluated employing a cross-type channel geometry. With the preferred sample loading method, the volume of the sample plug can be accurately controlled and is time independent, enabling a constant volume to be injected. The injection can be controlled electronically and does not induce any electrophoretic mobility based bias. Separations were performed on a compact chip with a serpentine column geometry that has a 165-mm separation channel in an area of less than 10 mm x 10 mm. Band-broadening effects in a serpentine column pattern were studied. 18 refs., 9 figs.

• DOE Contract Number: AC05-84OR21400
• OSTI ID: 7166277
• Journal Information: Analytical Chemistry (Washington); (United States), Journal Name: Analytical Chemistry (Washington); (United States) Vol. 66:7; ISSN 0003-2700; ISSN ANCHAM
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105* -- Separation Procedures
400400 -- Electrochemistry
400500 -- Photochemistry
99 GENERAL AND MISCELLANEOUS
990200 -- Mathematics & Computers
AMINES
AMMONIUM COMPOUNDS
AMMONIUM FLUORIDES
AMMONIUM HALIDES
CARBOXYLIC ACIDS
DYES
ELECTROPHORESIS
ETCHING
FLUORIDES
FLUORINE COMPOUNDS
GLASS
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROFLUORIC ACID
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
INORGANIC ACIDS
MATHEMATICAL MODELS
MINERALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDE MINERALS
OXYGEN COMPOUNDS
PERFORMANCE
PEROXIDES
QUARTZ
REAGENTS
RHODAMINES
SURFACE FINISHING