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Title: Precolumn reactions with electrophoretic analysis integrated on a microchip

Abstract

A glass microchip was constructed to perform chemical reactions and capillary electrophoresis sequentially. The channel manifold on the glass substrate was fabricated using standard photolithographic, etching, and deposition techniques. The microchip has a reaction chamber with a 1 nL reaction volume and a separation column with a 15.4 mm separation length. Electrical control of the buffer, analyte, and reagent streams made possible the precise manipulation of the fluids within the channel manifold. The microchip was operated under a continuous reaction mode with gated injections to introduce the reaction product onto the separation column with high reproducibility (<1.8% rsd in peak area). The reaction and separation performances were evaluated by reacting amino acids with o-phthaldialdehyde to generate a fluorescent product which was detected by laser-induced fluorescence. Control of the reaction and separation conditions was sufficient to measure reaction kinetics and variation of detection limits with reaction time. Half-times of reaction of 5.1 and 6.2 s and detection limits of 0.55 and 0.83 fmol were measured for arginine and glycine, respectively. 18 refs., 10 figs.

Authors:
; ; ;  [1]
  1. (Oak Ridge National Lab., TN (United States))
Publication Date:
OSTI Identifier:
6732069
DOE Contract Number:
AC05-84OR21400
Resource Type:
Journal Article
Resource Relation:
Journal Name: Analytical Chemistry (Washington); (United States); Journal Volume: 66:23
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; ALDEHYDES; CHEMICAL REACTIONS; AMINO ACIDS; DERIVATIZATION; AROMATICS; GLASS; ETCHING; ARGININE; CAPILLARIES; CHEMICAL ANALYSIS; CHEMICAL REACTION KINETICS; CHROMATOGRAPHY; COMPUTERS; ELECTRIC FIELDS; ELECTROPHORESIS; FLUORESCENCE; GLYCINE; LASERS; MICROANALYSIS; BLOOD VESSELS; BODY; CARBOXYLIC ACIDS; CARDIOVASCULAR SYSTEM; KINETICS; LUMINESCENCE; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; REACTION KINETICS; SEPARATION PROCESSES; SURFACE FINISHING 400100* -- Analytical & Separations Chemistry; 400201 -- Chemical & Physicochemical Properties; 990200 -- Mathematics & Computers

Citation Formats

Jacobson, S.C., Hergenroeder, R., Moore, A.W. Jr., and Ramsey, J.M.. Precolumn reactions with electrophoretic analysis integrated on a microchip. United States: N. p., 1994. Web. doi:10.1021/ac00095a003.
Jacobson, S.C., Hergenroeder, R., Moore, A.W. Jr., & Ramsey, J.M.. Precolumn reactions with electrophoretic analysis integrated on a microchip. United States. doi:10.1021/ac00095a003.
Jacobson, S.C., Hergenroeder, R., Moore, A.W. Jr., and Ramsey, J.M.. 1994. "Precolumn reactions with electrophoretic analysis integrated on a microchip". United States. doi:10.1021/ac00095a003.
@article{osti_6732069,
title = {Precolumn reactions with electrophoretic analysis integrated on a microchip},
author = {Jacobson, S.C. and Hergenroeder, R. and Moore, A.W. Jr. and Ramsey, J.M.},
abstractNote = {A glass microchip was constructed to perform chemical reactions and capillary electrophoresis sequentially. The channel manifold on the glass substrate was fabricated using standard photolithographic, etching, and deposition techniques. The microchip has a reaction chamber with a 1 nL reaction volume and a separation column with a 15.4 mm separation length. Electrical control of the buffer, analyte, and reagent streams made possible the precise manipulation of the fluids within the channel manifold. The microchip was operated under a continuous reaction mode with gated injections to introduce the reaction product onto the separation column with high reproducibility (<1.8% rsd in peak area). The reaction and separation performances were evaluated by reacting amino acids with o-phthaldialdehyde to generate a fluorescent product which was detected by laser-induced fluorescence. Control of the reaction and separation conditions was sufficient to measure reaction kinetics and variation of detection limits with reaction time. Half-times of reaction of 5.1 and 6.2 s and detection limits of 0.55 and 0.83 fmol were measured for arginine and glycine, respectively. 18 refs., 10 figs.},
doi = {10.1021/ac00095a003},
journal = {Analytical Chemistry (Washington); (United States)},
number = ,
volume = 66:23,
place = {United States},
year = 1994,
month =
}