Precolumn reactions with electrophoretic analysis integrated on a microchip

Journal Article · Thu Dec 01 04:00:00 EST 1994 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00095a003· OSTI ID:6732069

Jacobson, S C; Hergenroeder, R; Moore, Jr, A W; Ramsey, J M ^[1]

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1. Oak Ridge National Lab., TN (United States)

A glass microchip was constructed to perform chemical reactions and capillary electrophoresis sequentially. The channel manifold on the glass substrate was fabricated using standard photolithographic, etching, and deposition techniques. The microchip has a reaction chamber with a 1 nL reaction volume and a separation column with a 15.4 mm separation length. Electrical control of the buffer, analyte, and reagent streams made possible the precise manipulation of the fluids within the channel manifold. The microchip was operated under a continuous reaction mode with gated injections to introduce the reaction product onto the separation column with high reproducibility (<1.8% rsd in peak area). The reaction and separation performances were evaluated by reacting amino acids with o-phthaldialdehyde to generate a fluorescent product which was detected by laser-induced fluorescence. Control of the reaction and separation conditions was sufficient to measure reaction kinetics and variation of detection limits with reaction time. Half-times of reaction of 5.1 and 6.2 s and detection limits of 0.55 and 0.83 fmol were measured for arginine and glycine, respectively. 18 refs., 10 figs.

• DOE Contract Number: AC05-84OR21400
• OSTI ID: 6732069
• Journal Information: Analytical Chemistry (Washington); (United States), Journal Name: Analytical Chemistry (Washington); (United States) Vol. 66:23; ISSN 0003-2700; ISSN ANCHAM
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400100* -- Analytical &amp; Separations Chemistry
400201 -- Chemical &amp; Physicochemical Properties
99 GENERAL AND MISCELLANEOUS
990200 -- Mathematics &amp; Computers
ALDEHYDES
AMINO ACIDS
ARGININE
AROMATICS
BLOOD VESSELS
BODY
CAPILLARIES
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHEMICAL ANALYSIS
CHEMICAL REACTION KINETICS
CHEMICAL REACTIONS
CHROMATOGRAPHY
COMPUTERS
DERIVATIZATION
ELECTRIC FIELDS
ELECTROPHORESIS
ETCHING
FLUORESCENCE
GLASS
GLYCINE
KINETICS
LASERS
LUMINESCENCE
MICROANALYSIS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
REACTION KINETICS
SEPARATION PROCESSES
SURFACE FINISHING