Microchip capillary electrophoresis with an integrated postcolumn reactor

Journal Article · Sat Oct 15 04:00:00 EDT 1994 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00092a027· OSTI ID:7071695

Jacobson, S C; Koutny, L B; Hergenroeder, R; Moore, Jr, A W; Ramsey, J M ^[1]

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1. Oak Ridge National Lab., TN (United States)

A glass microchip with a postcolumn reactor was fabricated to conduct postseparation derivatization using o-phthaldialdehyde as a fluorescent [open quotes]tag[close quotes] for amino acids. This miniaturized separation device was constructed using standard photolithographic, wet chemical etching, and bonding techniques. Effects of the reagent stream on separation efficiency were investigated. In addition, a novel gated injector was demonstrated which maintains the integrity of the analyte, buffer, and reagent streams. 22 refs., 5 figs., 1 tab.

• DOE Contract Number: AC05-84OR21400
• OSTI ID: 7071695
• Journal Information: Analytical Chemistry (Washington); (United States), Journal Name: Analytical Chemistry (Washington); (United States) Vol. 66:20; ISSN 0003-2700; ISSN ANCHAM
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
400201 -- Chemical & Physicochemical Properties
ALDEHYDES
AMINO ACIDS
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHEMICAL REACTORS
DERIVATIZATION
ELECTROPHORESIS
FLUORESCENCE
LUMINESCENCE
ORGANIC ACIDS
ORGANIC COMPOUNDS
SEPARATION PROCESSES