Open channel electrochromatography on a microchip

Journal Article · Fri Jul 15 04:00:00 EDT 1994 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00086a024· OSTI ID:7016401

Jacobson, S C; Hergenroeder, R; Koutny, L B; Ramsey, J M ^[1]

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1. Oak Ridge National Lab., TN (United States)

A glass microchip having a channel with a cross section of 5.6 [mu]m high and 66 [mu]m wide was fabricated using standard photolithographic and etching techniques. The surface of the channel was chemically modified with octadecylsilane to function as the stationary phase for open channel chromatography. Electroosmotic flow was used to [open quotes]load[close quotes] the sample into the microchip and to [open quotes]pump[close quotes] the mobile phase during the experiments. For electric field strengths in the separation column from 27 to 163 V/cm, the linear velocity for the electroosmotic flow ranged from 0.13 to 0.78 mm/s. Detection was performed using direct fluorescence for separation monitoring and indirect fluorescence for void time measurements. Plate heights as low as 4.1 and 5.0 [mu]m were generated for unretained and retained components, respectively. 28 refs., 6 figs., 2 tabs.

• DOE Contract Number: AC05-84OR21400
• OSTI ID: 7016401
• Journal Information: Analytical Chemistry (Washington); (United States), Journal Name: Analytical Chemistry (Washington); (United States) Vol. 66:14; ISSN 0003-2700; ISSN ANCHAM
• Country of Publication: United States
• Language: English

Related Subjects

36 MATERIALS SCIENCE
360601 -- Other Materials-- Preparation & Manufacture
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
CHROMATOGRAPHY
COMPILED DATA
DATA
DIFFUSION
ELECTRIC FIELDS
ETCHING
FLUORESCENCE
GLASS
HYDRIDES
HYDROGEN COMPOUNDS
INFORMATION
LUMINESCENCE
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC SILICON COMPOUNDS
OSMOSIS
SEPARATION PROCESSES
SILANES
SILICON COMPOUNDS
SURFACE FINISHING
SURFACE TREATMENTS