Isolation of simian virus 40-transformed inbred hamster cell lines heterogeneous for virus induction by chemicals or radiation. [UV and gamma radiation, BUDR, mitomycin C]

Journal Article · Sat Nov 01 04:00:00 EST 1975 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(75)90161-0· OSTI ID:7365183

Kaplan, J C; Wilbert, S M; Collins, J J; Rakusanova, T; Zamansky, G B; Black, P H

Cloned cell lines have been isolated after simian virus 40 (SV40) transformation of kidney cells of an inbred hamster strain. Considerable heterogeneity for the induction of infectious virus was observed between the lines, ranging from the spontaneous production of infectious virus to nonproducer characteristics. In spite of their differences in virus inducibility, all the clones were found to contain equivalent small numbers of SV40 genomes (1-2) per cell as measured by C/sub 0/t analysis. The induction of infectious virus was studied after treating these clones with four agents that cause strand breakage either directly or indirectly in DNA: mitomycin C, BrdU and visible light, uv irradiation or /sup 60/Co ..gamma..-irradiation. A direct dose-response relationship was established between virus yield and dose of inducing agent used. Yields of infectious virus in induced cells were increased by factors of 10/sup 3/-10/sup 4/ compared to untreated cultures. In producer cell lines the percentage of induced cells was estimated to be 2-6 percent as determined by V antigen production or infectious center formation after treatment with the most effective inducing agent, mitomycin C. Our studies suggest that DNA damage and single-strand break formation may be early events in the induction of infectious SV40 from transformed cells by chemical and physical agents.

• Research Organization: Harvard Medical School, Boston
• OSTI ID: 7365183
• Journal Information: Virology; (United States), Journal Name: Virology; (United States) Vol. 68:1; ISSN VIRLA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBIOTICS
ANTIGENS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BROMOURACILS
BUDR
DNA
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTROMAGNETIC RADIATION
HAMSTERS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INFECTIVITY
MAMMALS
MICROORGANISMS
MITOMYCIN
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RIBOSIDES
RODENTS
STRAND BREAKS
ULTRAVIOLET RADIATION
URACILS
VERTEBRATES
VIRUSES
VISIBLE RADIATION