Replication of simian virus 40 in simian virus 40-transformed hamster kidney cells induced by mitomycin C or /sup 60/Co. gamma. irradiation

Journal Article · Sat Jul 15 04:00:00 EDT 1978 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(78)90287-8· OSTI ID:6661185

Rakusanova, T; Smales, W P; Kaplan, J C; Black, P H

Several clones of simian virus 40 (SV40)-transformed hamster kidney cells, which are heterogeneous for induction of infectious SV40, have been studied. SV40 yields are low after induction with /sup 60/Co ..gamma.. irradiation or mitomycin C. In order to clarify the mechanism(s) by which virus is produced in induced cells, we analyzed the replication of viral DNA and production of virion (V) antigen and infectious virus after induction in various clones as well as in lytically infected permissive cells. Cells replicating SV40 DNA or synthesizing V antigen were visualized by in situ hybridization and immunofluorescence techniques, respectively. Only some cells in induced cultures were found to produce SV40 and those which did were less efficient than lytically infected monkey cells. Mitomycin C or /sup 60/Co ..gamma.. irradiation acted by inducing more cells to replicate virus rather than by increasing the amount of SV40 released from individual cells. A greater proportion of cells could be induced to replicate SV40 DNA than to synthesize V antigen in all induced clones studied. Also, SV40 DNA replication was induced at lower doses of ..gamma.. irradiation than the production of either V antigen or infectious virus suggesting that synthesis of late virus protein is more restricted in induced cells than is replication of SV40 DNA. These findings indicate that one of the effects of induction treatments on SV40-transformed hamster cells is an enhancement of the cells' capacity to support SV40 replication.

• Research Organization: Massachusetts General Hospital, Boston
• OSTI ID: 6661185
• Journal Information: Virology; (United States), Journal Name: Virology; (United States) Vol. 88:2; ISSN VIRLA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBIOTICS
ANTIGENS
ANTIMITOTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL CULTURES
CELL PROLIFERATION
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
HAMSTERS
IONIZING RADIATIONS
KIDNEYS
MAMMALS
MICROORGANISMS
MITOMYCIN
MONKEYS
NUCLEIC ACID REPLICATION
ORGANS
PARASITES
PRIMATES
RADIATION EFFECTS
RADIATIONS
RODENTS
VERTEBRATES
VIRUSES