Tumor promoter 12-O-tetradecanoylphorbol 13-acetate stimulates simian virus 40 induction by DNA-damaging agents and tumor initiators
Simian virus 40 (SV40)-transformed Syrian hamster kidney cells produce infectious SV40 virus particles after treatments which damage DNA, such as UV irradiation or mitomycin C treatment. We have found that the induction of SV40 by DNA-damaging agents is greatly stimulated when a typical tumor promoter, 12-O-tetradecanoylphorbol 13-acetate (TPA), is present in the medium. Phorbol, which has a molecular structure similar to TPA but does not have any tumor-promoting activity, showed no such stimulatory effect on SV40 induction. This apparent synergistic effect of DNA-damaging agents and tumor promoter (TPA) was more pronounced when a tumor initiator, benzo (a)pyrene or 2-acetamido-fluorene, was combined with TPA. The effect of TPA on UV-triggered SV40 induction was greatly influenced by the timing of TPA addition to the culture medium, which was most efficient when addition of TPA was 5 to 20 h before UV irradiation. The effect of TPA, however, was not observed in SV40 rescue from hamster cells by cell fusion with permissive monkey (C7) cells.
- Research Organization:
- Univ. of Tokyo, Japan
- OSTI ID:
- 6723348
- Journal Information:
- Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 3:5; ISSN MCEBD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARCINOGENS
CONDENSED AROMATICS
DNA
DRUGS
ELECTROMAGNETIC RADIATION
ESTERS
HAMSTERS
HYDROCARBONS
MAMMALS
MICROORGANISMS
MITOMYCIN
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHORBOL ESTERS
PROMOTERS
RADIATION EFFECTS
RADIATIONS
RODENTS
SIMIAN VIRUS
SYNERGISM
TUMOR PROMOTERS
ULTRAVIOLET RADIATION
VERTEBRATES
VIRUSES