Cell cycle dependence of simian virus 40 induction from transformed hamster cells by ultraviolet irradiation
Journal Article
·
· Virology; (United States)
The induction of infectious simian virus 40 (SV40) was studied in synchronized cultures of SV40-transformed hamster cells. Cells were synchronized with a double excess thymidine block and induced by mitomycin C treatment or uv irradiation at hourly intervals after release from the block. Infectious virus was measured in cell-free extracts at 72 hr after initiating induction. The results indicated that virus activation by these agents was restricted to certain phases of the cell cycle. Mitomycin C was most effective if it was applied to cells 4 hr after the peak of DNA synthesis. With uv radiation, virus production was maximal if cells were irradiated 1-2 hr after the peak of DNA synthesis during the late S-G/sub 2/ phase of the cell cycle. The most sensitive period of the cell cycle for virus activation by uv is the one in which eukaryotic cells are most resistant to uv radiation, as measured by enhanced survival after irradiation. Therefore, it is possible that cellular DNA repair processes are more active during this inverval, promoting DNA strand break formation and viral genome excision.
- Research Organization:
- Harvard Medical School, Boston
- OSTI ID:
- 7286011
- Journal Information:
- Virology; (United States), Journal Name: Virology; (United States) Vol. 68:1; ISSN VIRLA
- Country of Publication:
- United States
- Language:
- English
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Tumor promoter 12-O-tetradecanoylphorbol 13-acetate stimulates simian virus 40 induction by DNA-damaging agents and tumor initiators
Journal Article
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Sat Jul 15 00:00:00 EDT 1978
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OSTI ID:6661185
Isolation of simian virus 40-transformed inbred hamster cell lines heterogeneous for virus induction by chemicals or radiation. [UV and gamma radiation, BUDR, mitomycin C]
Journal Article
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Fri Oct 31 23:00:00 EST 1975
· Virology; (United States)
·
OSTI ID:7365183
Tumor promoter 12-O-tetradecanoylphorbol 13-acetate stimulates simian virus 40 induction by DNA-damaging agents and tumor initiators
Journal Article
·
Sun May 01 00:00:00 EDT 1983
· Mol. Cell. Biol.; (United States)
·
OSTI ID:6723348
Related Subjects
560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBIOTICS
ANTIMITOTIC DRUGS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
CELL CYCLE
CHEMICAL ACTIVATION
DRUGS
ELECTROMAGNETIC RADIATION
HAMSTERS
HETEROCYCLIC COMPOUNDS
MAMMALS
MICROORGANISMS
MITOMYCIN
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOACTIVATION
RIBOSIDES
RODENTS
SYNCHRONOUS CULTURES
THYMIDINE
ULTRAVIOLET RADIATION
VERTEBRATES
VIRUSES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBIOTICS
ANTIMITOTIC DRUGS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
CELL CYCLE
CHEMICAL ACTIVATION
DRUGS
ELECTROMAGNETIC RADIATION
HAMSTERS
HETEROCYCLIC COMPOUNDS
MAMMALS
MICROORGANISMS
MITOMYCIN
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOACTIVATION
RIBOSIDES
RODENTS
SYNCHRONOUS CULTURES
THYMIDINE
ULTRAVIOLET RADIATION
VERTEBRATES
VIRUSES