Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Production, purification and assay of thrombopoietin. Progress report, June 1, 1973--Julty 31, 1976. [Mice, /sup 35/S tracer technique, X radiation]

Technical Report ·
OSTI ID:7362339
The finding that kidney cells in culture can produce thrombopoietin provides a technique whereby large quantites of thrombopoietin can be produced and may provide the necessary preliminary data for the production of an International Thrombopoietin Laboratory Standard. Studies showed that human urine, as well as sera, contains the thrombopoietic factor. Other studies indicated that the kidney is at least involved in the production of thrombopoietin and may be the site of its production. Additional work was involved with platelet-specific antiserum action and how it causes thrombocytopenia in animals. Two additional studies were conducted which were designed to improve the bioassay for TSF. In the first study, mice were pretreated with both platelet-specific antisera and platelet hypertransfusions in an attempt to increase their sensitivity to TSF. In another study, platelet size, platelet count, and percent /sup 35/S incorporation were compared as methods for the measurement of thrombopoietin-stimulated thrombopoiesis. The finding that thrombopoietin is present in sera of thrombocytopenic mice after either x-ray or injection of platelet-specific antisera, may offer a new approach to production and assay of TSF. Other studies during this grant period were involved with purification of thrombopoietin from kidney cell culture media. An investigation on the effects of hypoxia on platelet counts in mice was also made.
Research Organization:
Tennessee Univ., Knoxville (USA). Memorial Research Center and Hospital
OSTI ID:
7362339
Report Number(s):
ORO-4465-14
Country of Publication:
United States
Language:
English

Similar Records

Production, purification, and assay of thrombopoietin. Final report, June 1, 1973--June 30, 1978. [X radiation, mice, dogs]
Technical Report · Sat Dec 31 23:00:00 EST 1977 · OSTI ID:6893227
Production, purification and assay of thrombopoietin. Progress report, August 1, 1975--July 31, 1976. [X radiation, /sup 35/S]
Technical Report · Wed Dec 31 23:00:00 EST 1975 · OSTI ID:7195074
Bioassay for thrombopoietin utilizing mice in rebound thrombocytosis
Journal Article · Fri Nov 30 23:00:00 EST 1973 · Proc. Soc. Exp. Biol. Med., v. 144, no. 3, pp. 1006-1012 · OSTI ID:4429423

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANOXIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL WASTES
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BLOOD FORMATION
BLOOD PLATELETS
BLOOD SERUM
BODY
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
EVEN-ODD NUCLEI
HORMONES
IMMUNE SERUMS
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LIGHT NUCLEI
MAMMALS
MICE
NUCLEI
ORGANS
PURIFICATION
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RODENTS
STANDARDS
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
THROMBOPOIESIS
TRACER TECHNIQUES
URINE
VERTEBRATES
WASTES
X RADIATION