Bioassay for thrombopoietin utilizing mice in rebound thrombocytosis

Journal Article · Sat Dec 01 04:00:00 EST 1973 · Proc. Soc. Exp. Biol. Med., v. 144, no. 3, pp. 1006-1012

DOI:https://doi.org/10.3181/00379727-144-37730· OSTI ID:4429423

McDonald, T P

A bioassay for the detection of the thrombopoietic stimulating factor (TSF or thrombopoietin) that utilizes thrombocythemic mice has been presented. A single injection of anti-mouse platelet serum (AMPS) to mice produced a characteristic thrombocylopenia that was followed by rebound thrombocytosis. Two to three days later (7-- 8 days after the AMPS injection), a significantly (p < 0.05) depressed thrombopoiesis existed in response to the thrombocytosis. Normal and thrombocythemic mice were injected with normal sheep sera or sera from platelet-depleted sheep and Na/sub 2//sup 35/SO/sub 4/ i ncorporation into platelets was measured. A greater difference in /sp 35/S uptake existed between AMPS-treated control mice (mice injected with normal sheep serum) and AMPS- treated stimulated mice (mice injected with serum from platelet-depleted sheep) than in normal mice treated with the same sera. Also, TSF in sheep sera was shown to loss activity after storage at -2O deg . (auth)

• Research Organization: Univ. of Tennessee, Knoxville
• Sponsoring Organization: USDOE
• NSA Number: NSA-29-008176
• OSTI ID: 4429423
• Journal Information: Proc. Soc. Exp. Biol. Med., v. 144, no. 3, pp. 1006-1012, Journal Name: Proc. Soc. Exp. Biol. Med., v. 144, no. 3, pp. 1006-1012; ISSN PSEBA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

*HORMONES-- BIOASSAY
*SULFUR 35-- UPTAKE
BLOOD PLATELETS
BLOOD SERUM
MICE
N48830* --Life Sciences--New Tracer Techniques--Other
THROMBOPOIESIS
TRACER TECHNIQUES