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Production, purification and assay of thrombopoietin. Progress report, August 1, 1975--July 31, 1976. [X radiation, /sup 35/S]

Technical Report ·
OSTI ID:7195074
Experiments were conducted which indicate that thrombopoietin can be detected by both a bioassay and an immunoassay in sera of patients with various platelet production disorders. Other studies have shown that the kidney appears to have a vital role in thrombopoietin production; the mechanism of how platelet-specific antisera causes thrombocytopenia has been investigated. Also, an investigation has been made into the preparation of assay mice and the different methods for the measurement of thrombopoietin. These studies indicate that mice in rebound-thrombocytosis are more sensitive to exogenous TSF than normal or platelet transfused mice. Also, % /sup 35/S incorporation into platelets of assay mice is the most sensitive measurement of thrombopoiesis. The effects of hypoxia on platelet production was also investigated along with the release of thrombopoietin in animals exposed to RAMPS and whole-body x-irradiation.
Research Organization:
Tennessee Univ., Knoxville (USA). Memorial Research Center and Hospital
OSTI ID:
7195074
Report Number(s):
ORO-4465-15
Country of Publication:
United States
Language:
English

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Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANOXIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BLOOD FORMATION
BLOOD PLATELETS
BODY
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
EVEN-ODD NUCLEI
IONIZING RADIATIONS
ISOTOPES
KIDNEYS
LIGHT NUCLEI
MAMMALS
MEASURING METHODS
MICE
NUCLEI
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RODENTS
SULFUR 35
SULFUR ISOTOPES
THROMBOPOIESIS
VERTEBRATES
X RADIATION