Effects of x irradiation on respiratory control and P/O ratio in isolated rat liver mitochondria having different respiratory control ratios

Journal Article · Sat May 01 04:00:00 EDT 1976 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574408· OSTI ID:7362331

Atsuta, J; Okajima, S

The respiratory control and P/O ratios of isolated rat liver mitochondria held aerobically in buffered 0.3 M mannitol to 0.2 mM EDTA in an ice bath decreased exponentially immediately after x-irradiation at 20,000 R, in close correlation with the decrease of the preirradiation respiratory control. Of the two indicators of mitochondrial intactness, the respiratory control was the more sensitive to irradiation. This radiation effect appeared to be similar to uncoupling. The losses of respiratory control and P/O ratio in the irradiated mitochondria were amplified by transfer to ice-cold buffered 0.15 M mannitol without EDTA; the degree of this amplification reached a maximum for values of the preirradiation respiratory control ratio in the neighborhood of 4.0. The nature and direct cause of the radiation damage in mitochondria are discussed.

• Research Organization: Nagasaki Univ.
• OSTI ID: 7362331
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 66:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHELATING AGENTS
CONTROL
DIGESTIVE SYSTEM
EDTA
ELECTROMAGNETIC RADIATION
GLANDS
IONIZING RADIATIONS
LIVER
MAMMALS
METABOLISM
MITOCHONDRIA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
RADIATION EFFECTS
RADIATIONS
RATS
RESPIRATION
RODENTS
VERTEBRATES
X RADIATION