Uncoupling of oxidative phosphorylation in rat liver mitochondria by chloroethanols

Conference · Sun Mar 10 23:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5373738

Bhat, H K; Asimakis, G K; Anasari, G A.S. ^[1]

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1. Univ. of Texas Medical Branch, Galveston (United States)

Chloroethanols are toxic chemicals used in the industry and also formed as a result of the metabolism of several widely used halogenated hydrocarbons. The effect of 2-chloroethanol (CE), 2,2-dichloroethanol (DCE) and 2,2,2-trichloroethanol (TCE) on rat liver mitochondrial respiration was studied. Rat liver mitochondria were prepared in a mitochondrial isolation medium consisting of 250 mM sucrose, 10 mM Tris-HCl and 1 mM EDTA. Respiration of the mitochondrial suspension was determined with an oxygen electrode at 25C and the polarographic buffer consisted of 250 mM mannitol, 10 mM KCl, 10 mM K{sub 2}HPO{sub 4}, 5 mM MgCl{sub 2}, 0.2 mM EDTA and 10 mM Tris-HCl. With succinate as the respiratory substrate and using chloroethanols, CE stimulated respiration by 28.2 {plus minus} 6.5% and DCE by 202.7 {plus minus} 8.2% while TCE inhibited mitochondrial respiration. The effect of change in the concentration of chloroethanols on mitochondrial respiration was also studied. CE showed maximum stimulation at 600 mM, DCE at 150 mM and TCE at 30 mM. Respiratory stimulation was independent of mitochondrial protein concentration. Chloroethanols inhibited mitochondrial respiration when glutamate-malate was used as the respiratory substrate. Estimation of adenosine triphosphate (ATP) generation showed that chloroethanols inhibited the synthesis of ATP. These results indicate that chloroethanols stimulate mitochondrial respiration by uncoupling oxidative phosphorylation and that the uncoupling potency is proportional to the extent of chlorination.

• OSTI ID: 5373738
• Report Number(s): CONF-9104107--
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 5:4; ISSN 0892-6638; ISSN FAJOE
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
AROMATICS
ATP
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHELATING AGENTS
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
DIGESTIVE SYSTEM
EDTA
GLANDS
HALOGENATED AROMATIC HYDROCARBONS
INHIBITION
LIVER
MAMMALS
METABOLISM
MITOCHONDRIA
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANS
PHOSPHORYLATION
RATS
RESPIRATION
RODENTS
VERTEBRATES