Indirect Effect of X-Radiation on the Respiratory Metabolism of Ehrlich Ascites Tumour Cells and Mitochondria
Various proteinaceous and nonprotsiuaceous physiological media were incubated 3 hours at 37 deg C with mouse antero-latsral abdondnal wall tissue without radiation and with 1000 roentgen x radiation of the medium plus tissue prior to incubation. The respiratory and glycolytic metabolism of Ehrlich ascites cells and mitochondria was then compared in corresponding batches of media prepared with and without radiation. In some experiments oxidizable substrates were added to the media, while in others substrates liberated from the mouse abdominal wall tissue served to maintain respiration of the added ascites cells or mitochondria. With bicarborate buffering, intact ascites cells showed lower aerobic glycolysis and markedly elevated oxygen uptake in the x-irradiated medium. With phosphate buffering the opposite effect was observed. Both intact ascitte cells and mitochondria showed lower oxygen uptake in the x-irradiated msdium. Mitochondria showed lower P/O ratios in the x-irradiated msdium than in the corresponding non-irradiated control. Since neither the cells nor the mitochondria were directly radiated, the results demonstrate an indirect effect of x radiation on respiratory metabolism. The effect operates via the environmental medium irradiated in contact with a tissue selected to represent the normal tumor-bed and stroma of the Ehrlich tumor. The indirect effect does not seem due to radiolysis of the environmental medium itself, nor do organic hydroperoxides appear to be responsible. It is suggested that metabolic products from the irradiated tissue are liberated into the medium, and that these influence the respiration rate of added Ehrlich ascites cells and mitochondria.
- Research Organization:
- Liesbeek Cancer Research Clinic, Cape Town, South Africa
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-14-013597
- OSTI ID:
- 4173180
- Journal Information:
- British Journal of Cancer, Journal Name: British Journal of Cancer Journal Issue: 4 Vol. 13; ISSN 0007-0920
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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