Cellular heredity in haploid cultures of somatic cells. Annual progress report, March 1, 1975--March 31, 1976. [UV radiation]

Technical Report · Thu Jan 01 04:00:00 EST 1976

DOI:https://doi.org/10.2172/7356542· OSTI ID:7356542

Freed, J. J.

In experiments with haploid and diploid derivatives from the haploid frog embryo cell line ICR 2A, we have investigated aspects of cell survival, DNA repair and mutant induction after exposure to 254 nm radiation. Survival curves for haploid and diploid cells in random growth or blocked in the Gl phase of the cell cycle were determined; the survival data do not differ sufficiently to permit the use of such comparisons as an index of recessive lethal induction. Studies of the induction of thymine dimers in DNA indicated that the incidence of dimers in DNA from haploid and diploid cells is similar after exposure of the cells to equal doses of ultraviolet. The cells are capable of photoreversing dimers but appear to be deficient in excision repair. In an attempt to examine the effect of the permitted mode of DNA repair on the yield of mutations, we compared the incidence of ouabain-resistant variants among survivors of ultraviolet exposure and of ultraviolet exposure followed by photoreversal. Although the yield of resistant colonies was small, the data suggest that photoreversal lowers the yield of resistant colonies and thus that the induction of this phenotype is related to dimer persistence in DNA. We have also observed by fluorescence microscopy that an acridine mustard mutagen, ICR 191, is preferentially accumulated in cytoplasmic granules having the intracellular distribution pattern of lysosomes. This form of incorporation may be significant in the apparently non-genetic early toxicity of this compound observed in experiments with cultured cells.

• Research Organization: Institute for Cancer Research, Philadelphia, Pa. (USA)
• OSTI ID: 7356542
• Report Number(s): COO-3110-23
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGY
CELL CYCLE
CFU
DNA
ELECTROMAGNETIC RADIATION
EMBRYONIC CELLS
EMBRYOS
FROGS
GENETICS
MUTAGENS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLOIDY
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
SURVIVAL CURVES
TOXICITY
ULTRAVIOLET RADIATION
VERTEBRATES