Cellular heredity in haploid cultures of somatic cells. Progress report, August 1978-September 1979. [ICR 2A celline (frogs)]
During the past year, studies were continued on the relation of ultraviolet mutagenesis to DNA repair in cultures of the haploid frog cell line ICR 2A. The method of irradiation of cells in suspension was improved by construction of an improved detector with major sensitivity to the 254 nm Hg resonance line, to give better estimates of actual exposure of the cells. Using this method, dose-response and dose-fractionation studies on irradiation of ouabain resistance were carried out. The uv induction of this phenotype in the ICR 2A cell line was found to be less than that necessary for adequate analysis of dose-response curves. Cell fusion experiments using frog and mouse cells revealed an enhancement of mutagenesis in the mouse parent that will be explored in further work.
- Research Organization:
- Institute for Cancer Research, Philadelphia, PA (USA). Fox Chase Cancer Center
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC02-76EV03110
- OSTI ID:
- 5663415
- Report Number(s):
- DOE/EV/03110-31
- Country of Publication:
- United States
- Language:
- English
Similar Records
Cellular heredity in haploid cultures of somatic cells. Progress report, August 1978-September 1979. [Correlation between uv mutagenesis and DNA repair in cultured haploid frog cells (ICR 2A)]
Cellular heredity in haploid cultures of somatic cells. Annual progress report, March 1, 1975--March 31, 1976. [UV radiation]
Cellular heredity in haploid cultures of somatic cells, March 1968-April 1981. Final report
Technical Report
·
Mon Oct 01 00:00:00 EDT 1979
·
OSTI ID:5633971
Cellular heredity in haploid cultures of somatic cells. Annual progress report, March 1, 1975--March 31, 1976. [UV radiation]
Technical Report
·
Wed Dec 31 23:00:00 EST 1975
·
OSTI ID:7356542
Cellular heredity in haploid cultures of somatic cells, March 1968-April 1981. Final report
Technical Report
·
Sun Feb 28 23:00:00 EST 1982
·
OSTI ID:5028219
Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CORRELATIONS
DATA
DATA FORMS
DNA
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FRACTIONATED IRRADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HAPLOIDY
INFORMATION
IRRADIATION
ISOLATED VALUES
MUTAGENESIS
MUTATIONS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
PLOIDY
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
ULTRAVIOLET RADIATION
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CORRELATIONS
DATA
DATA FORMS
DNA
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FRACTIONATED IRRADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HAPLOIDY
INFORMATION
IRRADIATION
ISOLATED VALUES
MUTAGENESIS
MUTATIONS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
PLOIDY
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
ULTRAVIOLET RADIATION